Aminoallyl-UTP-ATTO-550

Aminoallyl-UTP-ATTO-550 is an aminoallyl-modified uridine triphosphate bearing an ATTO 550 fluorophore, enabling fluorescent nucleotide incorporation during nucleic acid labeling workflows. Researchers use its triphosphate functionality for polymerase-mediated incorporation and its aminoallyl handle for downstream conjugation strategies, supporting fluorescence microscopy and fluorescence-based nucleic acid analysis with an orange-red emitting dye.

1. Fluorescent RNA Labeling

Aminoallyl-UTP-ATTO-550 is widely used for fluorescent RNA labeling in transcription-based experiments, where it is incorporated into RNA by RNA polymerases to generate labeled transcripts for imaging and downstream characterization. Molecular biology and chemical biology labs often employ this reagent to prepare fluorescent RNA probes for localization studies, hybridization readouts, and assay development that require an ATTO 550 signal compatible with standard fluorescence microscopy filter sets and imaging workflows. The aminoallyl functionality provides a practical route for additional conjugation or crosslinking steps after incorporation, supporting flexible probe construction beyond "label-only" transcripts.

2. Nucleic Acid Hybridization Probes

Aminoallyl-UTP-ATTO-550 supports the preparation of fluorescent nucleic acid hybridization probes by enabling incorporation of ATTO 550 into RNA or RNA-like probe formats. In nucleic acid analysis workflows, researchers use these labeled probes to visualize target sequences in hybridization-based assays and to monitor binding events in fluorescence readouts. The triphosphate nature allows efficient incorporation during probe synthesis, while the aminoallyl group can be leveraged to tailor probe chemistry for specific assay formats, including conjugation to carriers or surfaces used in imaging and analytical platforms.

3. Fluorescent In Situ Hybridization

Aminoallyl-UTP-ATTO-550 is used in fluorescence-based in situ hybridization workflows to generate sequence-specific fluorescent signals for microscopy studies of nucleic acids in fixed samples. Cell biology and pathology-adjacent research groups commonly choose ATTO 550-labeled probe materials to obtain robust red/orange fluorescence for co-staining experiments and multi-channel imaging. By using polymerase-driven incorporation, the reagent helps produce probes that can be optimized for hybridization performance in microscopy workflows, while the aminoallyl handle offers additional flexibility for probe processing and conjugation when building complex staining panels.

4. Fluorescent Standards And Controls

Aminoallyl-UTP-ATTO-550 is also used to prepare fluorescent nucleic acid standards and assay controls, supporting method development in fluorescence imaging and nucleic acid quantification experiments. Laboratories developing hybridization protocols, imaging pipelines, or fluorescence-based readouts often rely on defined fluorescent nucleic acid materials to validate instrument settings, evaluate background contributions, and standardize labeling consistency across experiments. The ATTO 550 dye provides a convenient spectral marker for establishing reference signals, while the nucleotide incorporation strategy ensures the fluorescent label is embedded within a nucleic acid scaffold used in the assay workflow.