* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
AF488 is a bright green-fluorescent dye that is commonly used in microscopy and cell assays because of its photostability. AF488 can be used with DAPI and is well suited to multiplex assay. This high quantum yield dye has fluorescence stability within the pH range from 4 to 10.,AF488 carboxylic acid is a non-reactive AF488 form that can be used as a reference standard in experiments where AF488 conjugates are used. The carboxylic acid can be also used for the synthesis of activated esters [such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol) and STP (4-sulfo-2,3,5,6-tetrafluorophenol)] or modified with hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides. Thus, this derivative can be conjugated to molecules that contain amino groups, such as proteins, antibodies, and peptides. Therefore, AF488 carboxylic acid is used during solid-phase peptide synthesis for peptide modification in situ in the presence of activating agents such as HATU.
Chemical Information
Product Specification
Computed Properties
Purity |
NMR 1H, HPLC-MS (95%) |
IUPAC Name |
4-(3-amino-6-imino-4,5-disulfonatoxanthen-9-yl)-3-carboxybenzoate |
Canonical SMILES |
C1=CC(=C(C=C1C(=O)[O-])C(=O)O)C2=C3C=CC(=N)C(=C3OC4=C2C=CC(=C4S(=O)(=O)[O-])N)S(=O)(=O)[O-] |
InChI |
InChI=1S/C21H14N2O11S2/c22-13-5-3-10-15(9-2-1-8(20(24)25)7-12(9)21(26)27)11-4-6-14(23)19(36(31,32)33)17(11)34-16(10)18(13)35(28,29)30/h1-7,22H,23H2,(H,24,25)(H,26,27)(H,28,29,30)(H,31,32,33)/p-3 |
InChIKey |
IGAZHQIYONOHQN-UHFFFAOYSA-K |
Solubility |
Good in DMSO, DMF |
Appearance |
Orange crystals |
CF260 |
0.16 |
CF280 |
0.10 |
Fluorescence Quantum Yield |
0.91 |
Excitation |
495 |
Emission |
519 |
Storage |
Storage: 12 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
XLogP3 |
0.5 |
Hydrogen Bond Donor Count |
3 |
Hydrogen Bond Acceptor Count |
13 |
Rotatable Bond Count |
2 |
Exact Mass |
530.98042652 g/mol |
Monoisotopic Mass |
530.98042652 g/mol |
Topological Polar Surface Area |
268Ų |
Heavy Atom Count |
36 |
Formal Charge |
-3 |
Complexity |
1290 |
Isotope Atom Count |
0 |
Defined Atom Stereocenter Count |
0 |
Undefined Atom Stereocenter Count |
0 |
Defined Bond Stereocenter Count |
0 |
Undefined Bond Stereocenter Count |
0 |
Covalently-Bonded Unit Count |
1 |
Compound Is Canonicalized |
Yes |