7-(Diethylamino)coumarin-3-carboxylic Acid | 50995-74-9

7-(Diethylamino)coumarin-3-carboxylic Acid, a versatile fluorescent dye, renowned for its myriad applications in biological and chemical research. Here are four key applications:

Fluorescent Labeling: Widely utilized for labeling biomolecules such as proteins, nucleic acids, and lipids, 7-(Diethylamino)coumarin-3-carboxylic Acid shines in experimental setups. Its intense fluorescence facilitates the visualization and tracking of these molecules across diverse scenarios, playing a pivotal role in live-cell imaging, flow cytometry, and other fluorescence-based assays.

Biosensing: A game-changer in biosensor technology, this compound seamlessly detects a wide array of analytes, from ions to small organic molecules. The fluorescent properties of 7-(Diethylamino)coumarin-3-carboxylic Acid undergo a transformative shift upon binding to specific targets, enabling ultra-sensitive and highly targeted detection. These biosensors find utility in realms like medical diagnostics, environmental monitoring, and food safety assessments, showcasing their far-reaching impact.

Enzyme Assays: At the forefront of enzyme analysis, 7-(Diethylamino)coumarin-3-carboxylic Acid serves as a pivotal component in monitoring enzymatic activities. When linked to substrates, its fluorescent dye is released upon enzymatic cleavage, enabling precise quantitative measurement. This crucial application fuels investigations in enzyme kinetics, screening for enzyme inhibitors, and other intricate biochemical analyses, shedding light on the intricacies of enzymatic processes.

Chemical Probes: Researchers wield 7-(Diethylamino)coumarin-3-carboxylic Acid as a potent chemical probe to delve into diverse chemical landscapes and reactions. Its fluorescence dynamics exhibit sensitivity to factors like pH and polarity, making it a valuable tool for exploring chemical processes, designing drug delivery systems, and innovating novel materials, opening new horizons in chemical research.