7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester | 134471-24-2

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is a fluorescent compound widely utilized in biochemical and analytical applications. Here are some key applications of this compound:

Fluorescent Labeling: 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is extensively used for fluorescent labeling of proteins, peptides, and other biomolecules. By covalently attaching this ester to target molecules, researchers can visualize and track these molecules under a fluorescence microscope. This assists in studying protein localization, interactions, and dynamics in cellular environments.

Flow Cytometry: This compound is also employed in flow cytometry to label cells and analyze their properties. The fluorescent label provides a way to differentiate and sort cell populations based on specific markers or intracellular contents. This is important in clinical diagnostics, immunology research, and cell biology studies.

Enzyme Assays: 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is used in designing enzyme assays, where its fluorescence properties are exploited to measure enzymatic activity. When the labeled substrate is cleaved by the enzyme, changes in fluorescence intensity can be monitored. This allows for the quantitative analysis of enzyme kinetics and inhibitor screening.

Bioconjugation: In bioconjugation studies, the ester is utilized to link biomolecules with other functional groups for various applications, such as drug delivery and sensor development. The stable amide bond formed after conjugation ensures the integrity and functionality of the biomolecule. This expands the toolkit for creating novel biomaterials and investigative probes in biomedical research.