5-FAM-PEG3-DBCO

5-FAM-PEG3-DBCO, a versatile fluorescent probe, finds extensive use in bioconjugation and labeling applications. Here are four key applications:

Fluorescent Imaging: The widespread utilization of 5-FAM-PEG3-DBCO in cellular and tissue imaging is attributed to its remarkable fluorescent properties. By conjugating this probe to biomolecules like antibodies or peptides, researchers unlock the ability to visualize specific proteins or cellular structures under a fluorescence microscope. This aids in the examination of cellular dynamics, protein localization, and live-cell imaging, providing a window into the intricate world of cellular activities and interactions.

Bioconjugation: Within bioconjugation techniques, 5-FAM-PEG3-DBCO plays a pivotal role in connecting fluorescent labels to target biomolecules through the powerful tool of click chemistry. The DBCO group engages efficiently with azide-modified molecules, forging a stable triazole linkage. This click reaction boasts high specificity and unfolds under mild conditions, rendering it ideal for labeling proteins, nucleic acids, and other biomolecules in both research and diagnostic settings, propelling the field of bioconjugation into new realms of precision and utility.

Flow Cytometry: Embraced in the realm of flow cytometry, 5-FAM-PEG3-DBCO emerges as a cornerstone in staining cells for the analysis of surface or intracellular markers. By attaching the fluorescent probe to antibodies targeting specific cell surface proteins, researchers gain the ability to quantify and segregate diverse cell populations based on fluorescence intensity. This application stands as a linchpin for immunophenotyping, cell sorting, and functional investigations of immune cells, paving the way for deeper insights into cellular behavior and functionality.

FRET (Förster Resonance Energy Transfer) Studies: In the realm of FRET experiments, 5-FAM-PEG3-DBCO assumes a crucial role in probing molecular interactions and conformational changes. Teamed with a compatible FRET acceptor, this probe enables the detection of proximity-dependent energy transfer, unlocking insights into protein-protein interactions and molecular dynamics.