4,4-Difluoro-8-phenyl-3,5-diethyl-4-bora-3a,4a-diaza-s-indacene | CAS 1217340-97-0

4,4-Difluoro-8-phenyl-3,5-diethyl-4-bora-3a,4a-diaza-s-indacene is a boron-dipyrromethene (BODIPY) fluorescent dye designed for bright, photostable fluorescence labeling in chemical biology and materials research. Its rigid, conjugated fluorophore core supports efficient light emission, and the dye's organic-solvent compatibility makes it practical for preparing labeled probes, conjugates, and fluorescence standards used in microscopy and assay development.

1. Fluorescent Labeling Conjugates

4,4-Difluoro-8-phenyl-3,5-diethyl-4-bora-3a,4a-diaza-s-indacene is used as a fluorophore for constructing fluorescent conjugates for tracking biomolecular interactions and reaction workflows. Researchers incorporate the dye into labeling strategies for proteins, peptides, and other organic biomolecules to generate stable fluorescent reporters for imaging and plate-based readouts. In typical workflows, the dye is selected when a compact, BODIPY-type fluorophore is desired to support consistent fluorescence across labeling batches and downstream experimental handling.

2. Fluorescence Microscopy Imaging

4,4-Difluoro-8-phenyl-3,5-diethyl-4-bora-3a,4a-diaza-s-indacene supports fluorescence microscopy experiments where robust emission from a BODIPY fluorophore enables visualization of labeled targets and fluorescent tracers. Imaging teams use dye-labeled biomolecules or dye-doped polymer/biomaterial constructs to map localization, diffusion, and transport in prepared samples. The dye's strong fluorescence response is particularly useful for designing microscopy experiments where signal uniformity and reliable excitation/emission behavior are important for comparative imaging across conditions.

3. Fluorescence-Based Bioassays

4,4-Difluoro-8-phenyl-3,5-diethyl-4-bora-3a,4a-diaza-s-indacene is applied in fluorescence assay development as a labeling component for monitoring binding events, reagent mixing, and assay readouts. Assay developers use the dye to generate fluorescent standards and labeled reagents that integrate into common fluorescence plate formats, supporting quantitative measurement of fluorescence intensity changes associated with experimental endpoints. This use is common in research settings where a stable organic fluorophore is preferred for routine assay screening and method optimization.

4. Fluorescent Standards And Calibration

4,4-Difluoro-8-phenyl-3,5-diethyl-4-bora-3a,4a-diaza-s-indacene is frequently used to prepare fluorescence standards for instrument qualification and method calibration in fluorescence spectroscopy and imaging workflows. Laboratories rely on consistent dye emission to verify excitation alignment, monitor day-to-day instrument drift, and normalize fluorescence measurements between experiments. This application is especially relevant for teams building reproducible fluorescence data pipelines, where a well-behaved BODIPY fluorophore helps maintain comparability across imaging sessions and assay runs.