Naphthol AS-BI-Phosphate | CAS 1919-91-1

Catalog Number	A18-0022
Category	Fluorescent Enzyme Substrates
Molecular Formula	C18H15BrNO6P
Molecular Weight	452.2

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Product Introduction

Naphthol AS-BI-phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS-BI-phosphate is cleaved to release the fluorescent moiety naphthol AS-BI (N-ASBI), which can be used to quantify the activity of acid and alkaline phosphatases. Acid and alkaline phosphatases are commonly used for diagnosis of diseases.

Chemical Information
Product Specification
Application

Synonyms	Napthol AS-BI Phosphoric Acid; N-ASBI-P; 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-2-naphthalenecarboxamide; 6-Bromo-3-(o-hydroxyphenylaminocarbonyl)-2-naphthyl phosphate
Purity	≥95%
Canonical SMILES	COC1=CC=CC=C1NC(=O)C2=C(C=C3C=CC(=CC3=C2)Br)OP(=O)(O)O
InChI	InChI=1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
InChIKey	HUXIAXQSTATULQ-UHFFFAOYSA-N
Appearance	Solid Powder

Storage

Store at -20°C

Naphthol AS-BI-Phosphate is a substrate used primarily in biochemical research and diagnostic applications. Here are some key applications of Naphthol AS-BI-Phosphate:

Histochemistry: Naphthol AS-BI-Phosphate is widely used in histochemical staining to detect specific enzyme activities, such as alkaline phosphatase in tissue sections. The enzyme catalyzes the hydrolysis of Naphthol AS-BI-Phosphate, resulting in a colored precipitate that can be easily visualized under a microscope. This technique is essential for studying tissue morphology and identifying pathological changes.

Enzyme Assays: In biochemical research, Naphthol AS-BI-Phosphate serves as a chromogenic substrate for the quantitative measurement of phosphatase activity. Upon enzymatic hydrolysis, it produces a colored compound that can be measured spectrophotometrically. This application is crucial for assessing enzyme kinetics and inhibitor studies.

Immunoassays: Naphthol AS-BI-Phosphate can be used in ELISA (enzyme-linked immunosorbent assays) to detect the presence of phosphatase-conjugated antibodies. When the conjugated enzyme reacts with the substrate, a color change indicates the presence and quantity of the target antigen. This approach is valuable for diagnostic and research purposes in immunology and infectious disease.

Microbiology: In microbiology, Naphthol AS-BI-Phosphate can be incorporated into culture media to differentiate microbial species based on their phosphatase activity. Different microbes will hydrolyze the substrate at varying rates, leading to distinct color changes in the media. This is useful for identifying and characterizing different bacterial strains and their metabolic properties.