LDS 759 | CAS 133614-69-4

LDS 759 is a fluorescent dye designed for nucleic acid staining and fluorescence-based imaging workflows, providing strong contrast for DNA-rich samples when excited in the visible range. Its labeling performance is commonly leveraged in research settings where robust visualization of nucleic acids and nucleic-acid-containing structures is required, including gel and microscopy-style readouts.

1. DNA Staining In Gels

LDS 759 is used in electrophoresis workflows to visualize DNA-containing samples after separation, supporting routine gel imaging and documentation in molecular biology laboratories. Researchers typically incorporate the dye into post-staining or imaging steps to obtain clear fluorescence signals from nucleic-acid bands, enabling band identification and qualitative assessment of DNA distribution across lanes.

2. Fluorescence Microscopy Of Nuclei

LDS 759 supports fluorescence microscopy studies that require nucleic-acid contrast for cellular imaging, particularly when nuclei or DNA-rich compartments are the primary structures of interest. Cell biologists and imaging specialists use the dye as a DNA stain to visualize nuclear morphology and to support co-localization experiments with other fluorescent markers, using standard fluorescence microscope filter sets compatible with the dye's emission profile.

3. Flow Cytometry DNA Content

LDS 759 is applied in flow cytometry workflows where nucleic-acid-associated fluorescence is used to report DNA content in cell populations. Cytometry users integrate the dye into staining protocols for fluorescence readout on flow cytometers, enabling population-level analysis of DNA-associated signal distributions that are useful for cell-cycle-related studies and other nucleic-acid-dependent cytometry assays.

4. Fluorescent Nucleic Acid Assays

LDS 759 is also used in fluorescence-based nucleic acid assay development where DNA or DNA-containing complexes are monitored by fluorescence intensity. Molecular biology and chemical biology teams employ the dye to generate measurable fluorescence signals in assay formats that rely on nucleic-acid staining, supporting research investigations that require a straightforward fluorescent readout tied to DNA presence or abundance.