LDS 751 | 181885-68-7

LDS 751 is a specific type of cell-permeable fluorescent stain used primarily in the field of molecular biology to visualize nucleic acids. When LDS 751 binds to double-stranded DNA, it exhibits a significant increase in fluorescence intensity—approximately 20-fold. This ability to effectively highlight DNA makes it a crucial tool in various biological assays. Unlike some stains that can penetrate the nucleus, LDS 751 is known for its selective permeability, avoiding nuclear entry while effectively binding to polarized mitochondrial membranes. This unique property makes LDS 751 valuable for distinguishing cell organelles and DNA during fluorescence microscopy and other imaging techniques.

One of the key applications of LDS 751 is in flow cytometry, where it is utilized for the detection and analysis of nucleic acid content within cells. Its enhanced fluorescence upon DNA binding allows for the precise quantification of cellular DNA, aiding in the assessment of cell cycle phases and the identification of apoptotic cells. This capability is essential for research in cell biology and cancer studies, where understanding cell cycle dynamics is critical.

Another important application of LDS 751 is in confocal microscopy, which benefits from its high specificity and increased fluorescence to visualize cellular components. By selectively binding to mitochondria and other structures outside the nucleus, LDS 751 aids in the high-resolution imaging of these organelles, providing insights into their structure and function. This can be particularly useful in studying mitochondrial dynamics and dysfunctions linked to various diseases.

LDS 751 is also employed in multiplex assays, where its distinct spectral properties allow it to be used alongside other fluorescent stains. This capability ensures that researchers can simultaneously stain multiple targets within a single sample, leading to comprehensive analyses of complex cellular processes. The inclusion of LDS 751 in these assays increases the depth of data and enhances the understanding of interactions within the cellular environment.

Lastly, LDS 751 is valuable in live-cell imaging techniques, providing real-time observation of nucleic acid behavior within living cells. This application is particularly vital for studies requiring temporal analysis of cellular processes, such as the tracking of DNA replication or the investigation of mitochondrial activities under different physiological conditions. The stain’s cell-permeable nature and selective binding properties make it a favored choice for dynamic cellular investigations.