![{[Name]}](https://resource.bocsci.com/structure/1173109-53-9.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/150810-68-7.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/3548-09-2.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/5336-90-3.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/82855-40-1.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/57576-49-5.gif?v=2025011501)
Inquiry Basket
LDS 751 | CAS 181885-68-7
| Catalog Number | A19-0016 |
| Category | DNA Stains |
| Molecular Formula | C25H30ClN3O4 |
| Molecular Weight | 471.98 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
LDS 751 is a cell-permeable fluorescent stain for nucleic acid. Upon binding to double stranded DNA, the fluorescence of LDS 751 has increased 20-fold enhancement. Study shows that LDS-751 can be excluded from the nucleus and binds the polarized membranes of mitochondria.
Chemical Information
Application
| Synonyms | 6-(Dimethylamino)-2-{4-[4-(dimethylamino)phenyl]buta-1,3-dien-1-yl}-1-ethylquinolinium perchlorate |
| Canonical SMILES | CC[N+]1=C(C=CC2=C1C=CC(=C2)N(C)C)C=CC=CC3=CC=C(C=C3)N(C)C.[O-]Cl(=O)(=O)=O |
| InChI | InChI=1S/C25H30N3.ClHO4/c1-6-28-23(16-13-21-19-24(27(4)5)17-18-25(21)28)10-8-7-9-20-11-14-22(15-12-20)26(2)3;2-1(3,4)5/h7-19H,6H2,1-5H3;(H,2,3,4,5)/q+1;/p-1 |
| InChIKey | FGBAVQUHSKYMTC-UHFFFAOYSA-M |
| Appearance | Dark Brown to Black Solid |
LDS 751 is a specific type of cell-permeable fluorescent stain used primarily in the field of molecular biology to visualize nucleic acids. When LDS 751 binds to double-stranded DNA, it exhibits a significant increase in fluorescence intensity—approximately 20-fold. This ability to effectively highlight DNA makes it a crucial tool in various biological assays. Unlike some stains that can penetrate the nucleus, LDS 751 is known for its selective permeability, avoiding nuclear entry while effectively binding to polarized mitochondrial membranes. This unique property makes LDS 751 valuable for distinguishing cell organelles and DNA during fluorescence microscopy and other imaging techniques.
One of the key applications of LDS 751 is in flow cytometry, where it is utilized for the detection and analysis of nucleic acid content within cells. Its enhanced fluorescence upon DNA binding allows for the precise quantification of cellular DNA, aiding in the assessment of cell cycle phases and the identification of apoptotic cells. This capability is essential for research in cell biology and cancer studies, where understanding cell cycle dynamics is critical.
Another important application of LDS 751 is in confocal microscopy, which benefits from its high specificity and increased fluorescence to visualize cellular components. By selectively binding to mitochondria and other structures outside the nucleus, LDS 751 aids in the high-resolution imaging of these organelles, providing insights into their structure and function. This can be particularly useful in studying mitochondrial dynamics and dysfunctions linked to various diseases.
LDS 751 is also employed in multiplex assays, where its distinct spectral properties allow it to be used alongside other fluorescent stains. This capability ensures that researchers can simultaneously stain multiple targets within a single sample, leading to comprehensive analyses of complex cellular processes. The inclusion of LDS 751 in these assays increases the depth of data and enhances the understanding of interactions within the cellular environment.
Lastly, LDS 751 is valuable in live-cell imaging techniques, providing real-time observation of nucleic acid behavior within living cells. This application is particularly vital for studies requiring temporal analysis of cellular processes, such as the tracking of DNA replication or the investigation of mitochondrial activities under different physiological conditions. The stain’s cell-permeable nature and selective binding properties make it a favored choice for dynamic cellular investigations.
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products
Online Inquiry
