Hoechst 33258 analog | 258843-62-8

Catalog Number	A19-0063
Category	DNA Stains
Molecular Formula	C29H30N6O3
Molecular Weight	510.598

Catalog Number	Size	Price	Quantity
A19-0063	--	$--

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Product Introduction

An analog of Hoechst 33258. Hoechst 33258 is an iodinated DNA-binding bibenzimidazole, as well as a cell dye for DNA quantitation which sensitizes DNA and cells to UVA.

Chemical Information
Product Specification
Application

Synonyms	4-[3-[6-[6-(4-methylpiperazin-1-yl)-1-H-benzimidazol-2-yl]-1-H-benzimidazol-2-yl]phenoxy]butanoic acid
Shelf Life	2 years
Canonical SMILES	CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC(=CC=C6)OCCCC(=O)O
InChI	InChI=1S/C29H30N6O3/c1-34-11-13-35(14-12-34)21-8-10-24-26(18-21)33-29(31-24)20-7-9-23-25(17-20)32-28(30-23)19-4-2-5-22(16-19)38-15-3-6-27(36)37/h2,4-5,7-10,16-18H,3,6,11-15H2,1H3,(H,30,32)(H,31,33)(H,36,37)
InChI Key	QXQZJBBLMSBZHP-UHFFFAOYSA-N
Solubility	Soluble to ≥ 51 mg/mL in DMSO.
Density	1.333±0.06 g/cm3
Appearance	Light Yellow to Yellow Solid
Boiling Point	844.1±75.0 °C \| Condition: Press: 760 Torr

Storage

Store at -20°C

Hoechst 33258 analog, a bis-benzimide derivative, is widely utilized in biological research for its selective binding to the minor groove of DNA. Here are four key applications of Hoechst 33258 analog:

Nucleic Acid Staining: As a staple in cell biology and molecular biology, Hoechst 33258 analog is crucial for DNA staining applications. Its proficiency in illuminating cell nuclei under fluorescence microscopy provides researchers with a comprehensive view of cell morphology, cell cycle progression, and nuclear architecture. This selective binding property allows for precise and sensitive analysis of DNA dynamics within cellular structures.

Flow Cytometry: In the realm of flow cytometry, Hoechst 33258 analog plays a pivotal role in quantifying and evaluating DNA content at the individual cell level. By employing this dye to stain cells, researchers can delve into cell cycle dynamics and identify deviations in chromosomal DNA content. This application is particularly vital in cancer research for uncovering and comprehending the genetic variations across cell populations, contributing to advancements in understanding tumor biology.

Viability Assays: In the assessment of cell viability, Hoechst 33258 analog emerges as a powerful tool for distinguishing viable cells from those that are apoptotic or deceased. Its ability to permeate only cells with compromised membranes enables researchers to evaluate the integrity of cell membranes and overall cellular health with precision. This utility holds significant importance in drug screening processes and toxicological investigations, offering valuable insights into cellular responses to various stimuli.

Chromatin Structure Studies: Leveraging its unique binding characteristics, Hoechst 33258 analog stands out as an indispensable instrument for analyzing chromatin structure and dynamics. Researchers can harness its properties to explore the organization and compaction of chromatin under diverse physiological and pathological conditions. This investigation sheds light on gene regulation mechanisms and epigenetic modifications, enriching our understanding of the intricate interplay between chromatin structure and cellular function.

Applications of Fluorescent Probes & Dyes