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Fluorescein-5-thiosemicarbazide | CAS 76863-28-0
| Catalog Number | A16-0022 |
| Category | Other Cell Fluorescent Probes |
| Molecular Formula | C21H15N3O5S |
| Molecular Weight | 421.433 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Fluorescein-5-thiosemicarbazide (FTSC) is a fluorescent probe for labeling aldehydes and ketones in cell surface.
Chemical Information
Product Specification
Application
Computed Properties
Patents
| Synonyms | FTSC; 5-[(hydrazinylthioxomethyl)amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoic acid |
| IUPAC Name | 5-(aminocarbamothioylamino)-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid |
| Canonical SMILES | C1=CC(=C(C=C1NC(=S)NN)C(=O)O)C2=C3C=CC(=O)C=C3OC4=C2C=CC(=C4)O |
| InChI | InChI=1S/C21H15N3O5S/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19/h1-9,25H,22H2,(H,27,28)(H2,23,24,30) |
| InChIKey | MEUCHQDLZYLNQY-UHFFFAOYSA-N |
| Appearance | Solid Powder |
| Storage | Store at -20°C |
Fluorescein-5-thiosemicarbazide (FTSC) is a versatile fluorescent labeling reagent widely utilized in biochemical and cellular assays. Explore four key applications of FTSC:
Protein Labeling: Highly favored in the scientific realm for protein labeling, FTSC emerges as a prominent fluorescent tag, aiding in the detection and visualization of proteins across various analytical techniques. This strategic labeling technique empowers researchers to precisely trace the localization and interactions of proteins within cells using fluorescence microscopy. Moreover, quantifying labeled proteins in assays like flow cytometry and Western blotting enhances the depth of protein analysis, enriching protein characterization.
DNA and RNA Labeling: FTSC proves invaluable in labeling nucleic acids, enabling researchers to visually track DNA and RNA molecules during experimental procedures. This application holds particular significance in techniques such as in situ hybridization, where labeled nucleic acid probes selectively bind to specific sequences in chromosomes or tissue sections. The emitted fluorescence signal offers insights into gene expression patterns and the spatial arrangement of genetic material, unveiling intricate molecular processes.
Enzyme Assays: In the realm of enzyme assays, FTSC plays a pivotal role in synthesizing fluorescent substrates that allow real-time monitoring of enzymatic activity. Monitoring changes in fluorescence intensity enables researchers to delve into enzyme kinetics, evaluate inhibitor efficacy, and scrutinize the impact of varying conditions on enzyme performance. This pivotal information drives progress in basic research, refines drug development processes, and fosters innovation in enzymology.
Cell Viability and Proliferation Studies: Leveraging the adaptability of fluorescein-labeled molecules, researchers seamlessly incorporate FTSC-labeled reagents into living cells to assess cell viability and proliferation dynamics. These labeled reagents facilitate the staining of live cells, enabling researchers to monitor cell health and division patterns using techniques such as fluorescent microscopy or flow cytometry. This application is essential for toxicity screening, cancer research endeavors, and the exploration of cell cycle dynamics, contributing significantly to advancements in cellular biology and drug discovery initiatives.
| XLogP3 | -1 |
| Hydrogen Bond Donor Count | 5 |
| Hydrogen Bond Acceptor Count | 7 |
| Rotatable Bond Count | 3 |
| Exact Mass | 421.07324176 g/mol |
| Monoisotopic Mass | 421.07324176 g/mol |
| Topological Polar Surface Area | 166Ų |
| Heavy Atom Count | 30 |
| Formal Charge | 0 |
| Complexity | 852 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-114674734-A | A method for evaluating the antioxidant efficacy of cosmetic products and raw materials | 2022-04-20 |
| JP-2022162670-A | carbonylated proteolytic agent | 2021-04-13 |
| WO-2022185599-A1 | Carbonylated protein reducing agent for hair | 2021-03-01 |
| WO-2022145420-A1 | Novel multilayer polymer-coated crosslinked alginate gel fiber | 2020-12-28 |
| JP-2022102858-A | Weapon protein carbonylation inhibitor and external skin agent for keratin protein carbonylation inhibitor | 2020-12-25 |
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