DiD perchlorate | 127274-91-3
Catalog Number | A16-0008 |
Category | Cell membrane Fluorescent Probes |
Molecular Formula | C61H99ClN2O4 |
Molecular Weight | 959.9 |
Catalog Number | Size | Price | Quantity |
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A16-0008 | -- | $-- |
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Product Introduction
DiD perchlorate is a Cy5 dye used as a lipophilic fluorescent reagent.
Chemical Information |
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Synonyms | 2-[5-(1,3-Dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1,3-pentadien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indolium perchlorate; 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate; DilC18(5) dye; DilC18(5) |
Purity | ≥95% by HPLC |
Canonical SMILES | CCCCCCCCCCCCCCCCCCN1C2=CC=CC=C2C(C1=CC=CC=CC3=[N+](C4=CC=CC=C4C3(C)C)CCCCCCCCCCCCCCCCCC)(C)C.[O-]Cl(=O)(=O)=O |
InChI | InChI=1S/C61H99N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-35-44-52-62-56-48-42-40-46-54(56)60(3,4)58(62)50-38-37-39-51-59-61(5,6)55-47-41-43-49-57(55)63(59)53-45-36-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h37-43,46-51H,7-36,44-45,52-53H2,1-6H3;(H,2,3,4,5)/q+1;/p-1 |
InChI Key | ZQSBJPAQPRVNHU-UHFFFAOYSA-M |
- Product Specification
- Application
Excitation | 549 nm |
Emission | 565 nm |
Storage | Store at -20°C |
DiD perchlorate, a fluorescent dye known for its unique properties and versatility, finds widespread applications in various bioscience fields. Here are four key applications of DiD perchlorate:
Cell Membrane Labeling: Widely utilized in fluorescence microscopy and flow cytometry, DiD perchlorate serves as a potent tool for staining cell membranes. Its strong affinity for lipid bilayers allows researchers to visualize the dynamics and morphology of cell membranes with exceptional clarity. By tracking processes like endocytosis and apoptosis through labeled membranes, scientists gain valuable insights into cellular activities and behaviors.
Live Cell Imaging: Boasting high photostability and minimal cytotoxicity, DiD perchlorate excels in live cell imaging applications. This dye facilitates long-term tracking of cells in cultures or live tissues, providing crucial data for studies on cell migration, interactions, and tissue development. With its consistent fluorescence signal that resists photobleaching, researchers can continuously observe cells without compromising data integrity.
Multicolor Imaging: DiD perchlorate is a versatile component in multicolor imaging experiments when combined with other fluorescent dyes. Its unique emission spectrum allows for clear differentiation from other fluorophores, enabling simultaneous visualization of multiple cellular components or processes. This capability is indispensable in conducting complex biological analyses and co-localization studies that require precise identification and mapping of various biological entities.
Exosome Tracking: In the realm of exosome research, DiD perchlorate plays a crucial role in labeling these small extracellular vesicles involved in cell communication and biomarker investigations. By tagging exosomes with DiD, scientists can monitor their uptake and distribution in recipient cells or animal models, shedding light on the functions and implications of exosomes in physiological and pathological conditions.
Applications of Fluorescent Probes & Dyes
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