DDAO | 118290-05-4

DDAO (7-hydroxy-99-dimethyl-9H-acridin-2-one) is a fluorescent dye used extensively in various scientific applications due to its stability and unique spectral properties. Here are some key applications of DDAO:

Cellular Imaging: DDAO is employed in fluorescence microscopy to stain and visualize live and fixed cells due to its strong and stable fluorescence. It is particularly useful for tracing cellular components and tracking dynamic processes within cells. This application is essential for understanding cell morphology, function, and interactions in various biological contexts.

Protein Labeling: DDAO is often used for labeling proteins to study their localization, interactions, and functions. By conjugating DDAO with specific antibodies or proteins, researchers can detect and analyze protein expression within cells and tissues. This capability is vital for biochemical assays, immunoflu orescence, and other protein-based research methodologies.

Flow Cytometry: In flow cytometry, DDAO is utilized as a fluorescent marker to analyze the properties of individual cells within a heterogeneous population. Its distinctive emission wavelength allows for multiplexing with other fluorophores, providing comprehensive data on cell characteristics and surface markers. This application is crucial in immunology, cancer research, and cell sorting techniques.

Gene Expression Studies: DDAO can be used to label nucleic acids, enabling researchers to investigate gene expression patterns in various biological samples. By incorporating DDAO-labeled probes or primers in hybridization assays or real-time PCR, scientists can quantify and visualize specific genetic sequences. This application is instrumental in genomics, molecular diagnostics, and gene regulation studies.