Coumarin 521 | CAS 55804-67-6

Catalog Number	F06-0008
Category	Coumarin
Molecular Formula	C17H17NO3
Molecular Weight	283.32

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Product Introduction

Green fluorescent dye for microscopy, spectroscopy, and fluorescence calibration. Exhibits consistent brightness and solvent compatibility.

Chemical Information
Product Specification
Application

Synonyms	Coumarin 334;10-acetyl-2,3,6,7-tetrahydro-1H,5H,11H-[1]benzopyrano[6,7,8-ij]quinolizin-11-one; Coumarin 337
Canonical SMILES	CC(=O)C1=CC2=CC3=C4C(=C2OC1=O)CCCN4CCC3
InChI	InChI=1S/C17H17NO3/c1-10(19)14-9-12-8-11-4-2-6-18-7-3-5-13(15(11)18)16(12)21-17(14)20/h8-9H,2-7H2,1H3
InChIKey	JBPCDMSEJVCNGV-UHFFFAOYSA-N

Excitation	445
Emission	475
Storage	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Coumarin 521, a versatile fluorescent dye, finds wide-ranging utility in diverse scientific domains. Here are four key applications of Coumarin 521:

Cell Imaging: At the forefront of cell biology, Coumarin 521 plays a pivotal role in fluorescence microscopy by illuminating cellular structures. Through cell staining, researchers delve into dynamic processes like cell division and migration, unraveling the intricate tapestry of cellular functions and interactions in real-time.

Flow Cytometry: In the realm of flow cytometry, Coumarin 521 acts as a fluorescent beacon, labeling specific cell subsets for detailed analysis. This facilitates high-throughput examination of cellular characteristics such as size, complexity, and protein expression. Researchers leverage this data for immunophenotyping, cell sorting, and investigating cellular responses to diverse treatments.

Protein Labeling: Delving into the realm of protein dynamics, Coumarin 521 becomes instrumental by binding to proteins and unveiling their localization, interactions, and behaviors within living cells. Tracking these fluorescently labeled proteins sheds light on intricate protein functions and molecular pathways crucial for deciphering disease mechanisms and steering targeted therapy development.

Environmental Sensing: Extending its reach into environmental science, Coumarin 521 emerges as a potent tool for detecting and monitoring contaminants in water and soil. Its unique fluorescence properties respond sensitively to specific chemicals, serving as a beacon for environmental pollution assessment and guiding the formulation of effective remediation strategies.