BODIPY TR-X NHS Ester | CAS 217190-13-1

BODIPY TR-X NHS Ester is an NHS-ester activated BODIPY fluorophore designed for covalent fluorescent labeling of primary amine-containing biomolecules. Its red-emitting, high-brightness BODIPY core supports fluorescence microscopy and fluorescence-based assays, while the N-hydroxysuccinimide ester enables efficient conjugation under standard bioconjugation conditions. This reagent is commonly used when a stable, photostable red dye tag is needed for tracking, quantification, or imaging reagent development.

1. Protein And Antibody Labeling

BODIPY TR-X NHS Ester is frequently used by protein bioconjugation groups to generate red fluorescent conjugates for immunofluorescence workflows and antibody labeling. Researchers typically attach the dye to lysine residues on proteins, enabling consistent visualization of targets in cell-based assays and microscopy studies. Because the reagent is an NHS ester, it is also widely employed for preparing fluorescent secondary reagents and labeling standards used to compare staining performance across experiments.

2. Fluorescence Microscopy Staining

BODIPY TR-X NHS Ester supports fluorescence microscopy applications where red emission is advantageous for multiplexing or for separating signal from common autofluorescence backgrounds. Imaging teams use the dye-labeled biomolecules to stain extracellular targets, track binding interactions, or label biomolecular assemblies in fixed-sample workflows. The BODIPY fluorophore format is often selected in microscopy reagent development when robust fluorescence output is needed for multi-step staining and wash procedures.

3. Flow Cytometry Conjugate Preparation

BODIPY TR-X NHS Ester is used in flow cytometry reagent development to prepare fluorescently labeled proteins and affinity reagents that report on binding to cell-surface or extracellular components. Conjugation via NHS chemistry to amine-bearing biomolecules allows researchers to generate consistent fluorescent probes for panel development and quantitative fluorescence readouts. Laboratories often choose this dye when a red-channel fluorophore is required to complement existing green or far-red labels in multi-color cytometry experiments.

4. Fluorescent Assay Reagent Development

BODIPY TR-X NHS Ester is also applied to build fluorescent assay reagents for plate-based measurements, including labeling of enzymes, binding proteins, or assay components that require a stable red fluorophore tag. Assay developers use the NHS-ester chemistry to incorporate the dye into functional biomolecular conjugates used as signal reporters in binding studies, reagent tracking, and assay optimization experiments. The resulting BODIPY-labeled constructs are commonly integrated into fluorescence readouts where spectral separation and reliable emission from the red channel are important.