BODIPY TR methyl ester

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BODIPY TR methyl ester

BODIPY TR methyl ester | 150152-63-9

Catalog Number F01-0158
Category BODIPY
Molecular Formula C22H17BF2O3S
Molecular Weight 438.25
Catalog Number Size Price Quantity
F01-0158 -- $--

Product Introduction

BODIPY TR methyl ester is a cell-permeant, red-fluorescent dye that is used for staining organelles like mitochondria, endoplasmic reticulum, and Golgi apparatus. It has been used as a counterstain in cells or tissues expressing green fluorescent protein (GFP).

Chemical Information

Synonyms CellHunt EverFluor TR Methyl Ester; Lipophilic Counterstain For GFP; CellTrace BODIPY TR methyl ester
IUPAC Name methyl 2-[4-(2,2-difluoro-12-thiophen-2-yl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)phenoxy]acetate
Canonical SMILES [B-]1(N2C(=CC=C2C3=CC=CS3)C=C4[N+]1=C(C=C4)C5=CC=C(C=C5)OCC(=O)OC)(F)F
InChI InChI=1S/C22H17BF2N2O3S/c1-29-22(28)14-30-18-8-4-15(5-9-18)19-10-6-16-13-17-7-11-20(21-3-2-12-31-21)27(17)23(24,25)26(16)19/h2-13H,14H2,1H3
InChI Key HQMDVGPFFTVIIE-UHFFFAOYSA-N
  • Product Specification
  • Application
Excitation 494
Emission 517

BODIPY TR methyl ester, a highly fluorescent dye renowned for its superb photostability, finds widespread usage in diverse bioscience applications. Here are four key applications of BODIPY TR methyl ester:

Live Cell Imaging: Embedded in the realm of live cell imaging, BODIPY TR methyl ester emerges as a pivotal tool thanks to its prowess in staining cellular structures while upholding cell viability. Boasting exceptional photostability and intense fluorescence, this dye becomes the go-to option for monitoring dynamic processes within cells. Researchers can witness and track cellular events in real-time, unraveling profound insights into the intricate functionalities and behaviors of cells.

Fluorescence Microscopy: Within the domain of fluorescence microscopy, BODIPY TR methyl ester stands out as a dependable probe for labeling proteins, nucleic acids, and other vital biomolecules. Its sharp emission spectrum and vivid fluorescence not only amplify the contrast of microscopic images but also facilitate the examination of cellular structures and interactions at a nuanced, high-resolution level.

High-Throughput Screening: Serving as a cornerstone in high-throughput screening assays, BODIPY TR methyl ester assumes a crucial role due to its fluorescent attributes that enable swift and efficient testing of extensive compound libraries. The dye’s seamless integration with automation and detection systems streamlines the identification of active compounds, expediting the drug discovery process and enhancing the efficiency of various biochemical analyses.

Flow Cytometry: In the intricate world of flow cytometry, BODIPY TR methyl ester takes center stage as a labeling agent for cells, facilitating multiparametric analysis. Its radiant fluorescence simplifies the quantitative assessment of cell properties like size, structure, and marker expression. This application is imperative for tasks such as immunophenotyping, cell sorting, and a spectrum of diagnostic applications in both research settings and clinical practice.

Computed Properties

Hydrogen Bond Donor Count 0
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 6
Exact Mass 438.1021001 g/mol
Monoisotopic Mass 438.1021001 g/mol
Topological Polar Surface Area 71.7Ų
Heavy Atom Count 31
Formal Charge 0
Complexity 826
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 1
Compound Is Canonicalized Yes

Patents

Publication NumberTitlePriority Date
US-2022315614-A1 Reducing agents and uses thereof 2021-03-19
US-11174281-B1 Modified nucleotides and uses thereof 2020-04-24
WO-2021216998-A1 Modified nucleotides and uses thereof 2020-04-24
EP-4117682-A1 Modified nucleotides and uses thereof 2020-04-24
US-2023002436-A1 Modified nucleotides and uses thereof 2020-04-24
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