BODIPY TR methyl ester | 150152-63-9
Catalog Number | F01-0158 |
Category | BODIPY |
Molecular Formula | C22H17BF2O3S |
Molecular Weight | 438.25 |
Catalog Number | Size | Price | Quantity |
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F01-0158 | -- | $-- |
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Product Introduction
BODIPY TR methyl ester is a cell-permeant, red-fluorescent dye that is used for staining organelles like mitochondria, endoplasmic reticulum, and Golgi apparatus. It has been used as a counterstain in cells or tissues expressing green fluorescent protein (GFP).
Chemical Information |
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Synonyms | CellHunt EverFluor TR Methyl Ester; Lipophilic Counterstain For GFP; CellTrace BODIPY TR methyl ester |
IUPAC Name | methyl 2-[4-(2,2-difluoro-12-thiophen-2-yl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)phenoxy]acetate |
Canonical SMILES | [B-]1(N2C(=CC=C2C3=CC=CS3)C=C4[N+]1=C(C=C4)C5=CC=C(C=C5)OCC(=O)OC)(F)F |
InChI | InChI=1S/C22H17BF2N2O3S/c1-29-22(28)14-30-18-8-4-15(5-9-18)19-10-6-16-13-17-7-11-20(21-3-2-12-31-21)27(17)23(24,25)26(16)19/h2-13H,14H2,1H3 |
InChI Key | HQMDVGPFFTVIIE-UHFFFAOYSA-N |
- Product Specification
- Application
Excitation | 494 |
Emission | 517 |
BODIPY TR methyl ester, a highly fluorescent dye renowned for its superb photostability, finds widespread usage in diverse bioscience applications. Here are four key applications of BODIPY TR methyl ester:
Live Cell Imaging: Embedded in the realm of live cell imaging, BODIPY TR methyl ester emerges as a pivotal tool thanks to its prowess in staining cellular structures while upholding cell viability. Boasting exceptional photostability and intense fluorescence, this dye becomes the go-to option for monitoring dynamic processes within cells. Researchers can witness and track cellular events in real-time, unraveling profound insights into the intricate functionalities and behaviors of cells.
Fluorescence Microscopy: Within the domain of fluorescence microscopy, BODIPY TR methyl ester stands out as a dependable probe for labeling proteins, nucleic acids, and other vital biomolecules. Its sharp emission spectrum and vivid fluorescence not only amplify the contrast of microscopic images but also facilitate the examination of cellular structures and interactions at a nuanced, high-resolution level.
High-Throughput Screening: Serving as a cornerstone in high-throughput screening assays, BODIPY TR methyl ester assumes a crucial role due to its fluorescent attributes that enable swift and efficient testing of extensive compound libraries. The dye’s seamless integration with automation and detection systems streamlines the identification of active compounds, expediting the drug discovery process and enhancing the efficiency of various biochemical analyses.
Flow Cytometry: In the intricate world of flow cytometry, BODIPY TR methyl ester takes center stage as a labeling agent for cells, facilitating multiparametric analysis. Its radiant fluorescence simplifies the quantitative assessment of cell properties like size, structure, and marker expression. This application is imperative for tasks such as immunophenotyping, cell sorting, and a spectrum of diagnostic applications in both research settings and clinical practice.
Computed Properties | |
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Hydrogen Bond Donor Count | 0 |
Hydrogen Bond Acceptor Count | 7 |
Rotatable Bond Count | 6 |
Exact Mass | 438.1021001 g/mol |
Monoisotopic Mass | 438.1021001 g/mol |
Topological Polar Surface Area | 71.7Ų |
Heavy Atom Count | 31 |
Formal Charge | 0 |
Complexity | 826 |
Isotope Atom Count | 0 |
Defined Atom Stereocenter Count | 0 |
Undefined Atom Stereocenter Count | 0 |
Defined Bond Stereocenter Count | 0 |
Undefined Bond Stereocenter Count | 0 |
Covalently-Bonded Unit Count | 1 |
Compound Is Canonicalized | Yes |
Patents
Publication Number | Title | Priority Date |
---|---|---|
US-2022315614-A1 | Reducing agents and uses thereof | 2021-03-19 |
US-11174281-B1 | Modified nucleotides and uses thereof | 2020-04-24 |
WO-2021216998-A1 | Modified nucleotides and uses thereof | 2020-04-24 |
EP-4117682-A1 | Modified nucleotides and uses thereof | 2020-04-24 |
US-2023002436-A1 | Modified nucleotides and uses thereof | 2020-04-24 |
Applications of Fluorescent Probes & Dyes
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