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BODIPY
BODIPY FL Histamine

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BODIPY FL Histamine

Catalog Number	F01-0207
Category	BODIPY
Molecular Formula	C19H22BF2N5O
Molecular Weight	385.22

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Product Introduction

BODIPY FL Histamine is a cell-permeant, green-fluorescent dye that accumulates in acidic organelles of living cells. It has been used to label and track acidic organelles of living cells, and also used to investigate binding kinetics for the four histamine receptor subtypes by flow cytometry. This probe is relatively insensitive to environment and pH changes.

Chemical Information
Application

Synonyms	EverFluor FL Histamine
Appearance	Solid Powder

BODIPY FL Histamine is a fluorescent probe used in various scientific research applications, particularly in the study of histamine-related processes. Here are some key applications of BODIPY FL Histamine:

Cellular Imaging: BODIPY FL Histamine is used in cellular imaging to visualize and quantify histamine uptake and release in live cells. Its fluorescent properties allow researchers to track histamine dynamics with high sensitivity and specificity. This application is crucial for studying histamine’s role in physiological and pathophysiological conditions such as allergies and inflammation.

Receptor Binding Studies: In pharmacological research, BODIPY FL Histamine serves as a valuable tool for studying histamine receptor interactions. By labeling histamine with this fluorescent probe, researchers can monitor the binding affinity and kinetics of histamine to its receptors. This helps in understanding receptor function and developing drugs that target histamine pathways.

Flow Cytometry: BODIPY FL Histamine is employed in flow cytometry as a fluorescent marker to analyze histamine-related cellular responses. The probe’s fluorescence facilitates the sorting and quantification of cells based on their histamine levels. This technique is essential for identifying histamine-producing cells and understanding their role in immune and allergic responses.

Assay Development: In biochemical assays, BODIPY FL Histamine is used as a fluorescent tracer to measure histamine concentration and activity. The probe’s sensitivity allows for the accurate detection of histamine in various biological samples such as blood and tissue extracts. This application is beneficial for diagnostic purposes and high-throughput screening of histamine-related compounds.