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BODIPY
BODIPY FL-C5 NHS Ester

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BODIPY FL-C5 NHS Ester

Catalog Number	F01-0189
Category	BODIPY
Molecular Formula	C20H22BF2N3O4
Molecular Weight	417.21

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Product Introduction

BODIPY FL-C5 NHS Ester is a green-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. It is non-ionic, and relatively insensitive to environment and pH changes. The five-atom spacer between the dye and it's point of attachment may reduce interactions of the dye and the biomolecule to which it has reacted.

Chemical Information
Application

Synonyms	EverFluor FL-C5 NHS Ester (Succinimidyl Ester)
Appearance	Solid Powder

BODIPY FL-C5 NHS Ester is a reactive fluorescent dye widely used in various scientific applications due to its excellent photostability and brightness. Here are some key applications of BODIPY FL-C5 NHS Ester:

Fluorescent Labeling: BODIPY FL-C5 NHS Ester is commonly used for labeling proteins, peptides, and other biomolecules. It reacts with primary amines in biomolecules, forming a stable covalent bond that results in a bright fluorescent tag. This labeling is crucial for tracking and visualizing molecules in live cells or complex biological systems using fluorescence microscopy and flow cytometry.

Cellular Imaging: In cell biology, BODIPY FL-C5 NHS Ester is employed for imaging live and fixed cells. Its strong fluorescence allows for high-resolution imaging of cellular structures and processes. Researchers use it to study cell signaling, trafficking, and morphological changes, enhancing our understanding of cellular dynamics.

Flow Cytometry: BODIPY FL-C5 NHS Ester is used in flow cytometry for detecting and analyzing specific cell populations within a heterogeneous sample. By tagging antibodies or proteins with this dye, scientists can effectively measure cell surface markers or intracellular proteins. This application is essential in immunophenotyping, cell sorting, and diagnostic assays.

FRET Studies: BODIPY FL-C5 NHS Ester is utilized in Förster resonance energy transfer (FRET) studies to investigate molecular interactions and conformations. Its brightness and spectral properties make it suitable as a donor or acceptor in FRET pairs. These studies provide insights into protein-protein interactions, molecular folding, and the real-time dynamics of biological molecules.