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BODIPY 650/665-X SE
| Catalog Number | F01-0192 |
| Category | BODIPY |
| Molecular Formula | C33H32BF2N5O6 |
| Molecular Weight | 643.44 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 650/665-X SE is a reactive fluorescent dye belonging to the BODIPY class, known for its exceptional photostability and sharp emission peaks. It features an amine-reactive succinimidyl ester (SE) group, enabling efficient conjugation to proteins, peptides, and other biomolecules under mild conditions. This dye is frequently used in fluorescence imaging and flow cytometry applications, where its distinct spectral properties allow for precise multicolor labeling and detection.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | EverFluor 650/665-X SE; BODIPY 650/665-X NHS Ester |
| Appearance | Solid Powder |
Product Specification
| Excitation | 519 |
| Emission | 546 |
Application
BODIPY 650/665-X SE is a fluorescent dye commonly used in various biological research applications due to its exceptional photostability and brightness. Here are some key applications of BODIPY 650/665-X SE:
Fluorescence Microscopy: BODIPY 650/665-X SE is extensively used in fluorescence microscopy for imaging cellular structures and biomolecules. Its bright fluorescence and optimal spectral properties allow clear visualization in multicolor staining experiments. Researchers utilize this dye for studying cellular morphology, protein localization, and interactions at the microscopic level.
Flow Cytometry: In flow cytometry, BODIPY 650/665-X SE helps in the analysis of cell populations by labeling specific cellular components or markers. Due to its compatible emission spectrum, it integrates seamlessly with other common fluorophores, enabling multiplexing in immunophenotyping. This facilitates precise discrimination between different cell types and states in complex biological samples.
Fluorescent Assays: BODIPY 650/665-X SE is employed in various biochemical assays to detect and quantify biomolecule interactions. Its high sensitivity and stable fluorescence make it ideal for assays like enzyme activity measurements and receptor-ligand binding studies. These assays provide insights into molecular mechanisms and are crucial for drug discovery and development processes.
Live-Cell Imaging: BODIPY 650/665-X SE is suitable for live-cell imaging applications due to its low photobleaching and cell-permeable properties. It allows researchers to monitor dynamic processes such as intracellular trafficking, cell signaling, and division in real-time.
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