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BODIPY
BODIPY 650/665-X SE

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BODIPY 650/665-X SE

Catalog Number	F01-0192
Category	BODIPY
Molecular Formula	C33H32BF2N5O6
Molecular Weight	643.44

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* Please be kindly noted products are not for therapeutic use. We do not sell to patients.

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Product Introduction

BODIPY 650/665-X SE is a red-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. It is relatively insensitive to environment and pH changes. The seven-atom spacer between the dye and it's point of attachment may reduce interactions of the dye and the biomolecule to which it has reacted.

Chemical Information
Product Specification
Application

Synonyms	EverFluor 650/665-X SE; BODIPY 650/665-X NHS Ester
Appearance	Solid Powder

Excitation	519
Emission	546

BODIPY 650/665-X SE is a fluorescent dye commonly used in various biological research applications due to its exceptional photostability and brightness. Here are some key applications of BODIPY 650/665-X SE:

Fluorescence Microscopy: BODIPY 650/665-X SE is extensively used in fluorescence microscopy for imaging cellular structures and biomolecules. Its bright fluorescence and optimal spectral properties allow clear visualization in multicolor staining experiments. Researchers utilize this dye for studying cellular morphology, protein localization, and interactions at the microscopic level.

Flow Cytometry: In flow cytometry, BODIPY 650/665-X SE helps in the analysis of cell populations by labeling specific cellular components or markers. Due to its compatible emission spectrum, it integrates seamlessly with other common fluorophores, enabling multiplexing in immunophenotyping. This facilitates precise discrimination between different cell types and states in complex biological samples.

Fluorescent Assays: BODIPY 650/665-X SE is employed in various biochemical assays to detect and quantify biomolecule interactions. Its high sensitivity and stable fluorescence make it ideal for assays like enzyme activity measurements and receptor-ligand binding studies. These assays provide insights into molecular mechanisms and are crucial for drug discovery and development processes.

Live-Cell Imaging: BODIPY 650/665-X SE is suitable for live-cell imaging applications due to its low photobleaching and cell-permeable properties. It allows researchers to monitor dynamic processes such as intracellular trafficking, cell signaling, and division in real-time.