BODIPY 630/650 Acid

BODIPY 630/650 Acid is a fluorescent dye renowned for its stability and brightness in various applications. Here are some key applications of BODIPY 630/650 Acid:

Fluorescence Microscopy: BODIPY 630/650 Acid is widely used in fluorescence microscopy due to its exceptional photostability and high quantum yield. This dye provides sharp and bright images, making it ideal for live-cell imaging studies. Researchers can use it to track cellular processes such as protein localization and interaction with high sensitivity and precision.

Flow Cytometry: In flow cytometry, BODIPY 630/650 Acid serves as a reliable fluorescent label for analyzing cell populations. Its emission spectrum allows it to be distinguished from other commonly used dyes, enabling multiplexing in complex samples. This application is crucial for immunophenotyping, detecting fluorescently labeled antibodies or ligands that bind to specific cell surface markers.

Fluorescence Resonance Energy Transfer (FRET) Studies: BODIPY 630/650 Acid’s fluorescence properties make it suitable for FRET applications, which study molecular interactions and distances. By pairing it with suitable FRET partners, researchers can investigate conformational changes, protein-protein interactions, and nucleic acid dynamics in real-time. This provides invaluable insights into the molecular mechanisms underpinning biological functions.

Biochemical Assays: BODIPY 630/650 Acid is utilized in various biochemical assays to monitor enzyme activity, detect analytes, or measure protein concentrations. Its fluorescent properties make it a sensitive reporter for high-throughput screening assays in drug discovery. This application aids in uncovering novel compounds and understanding biochemical pathways relevant to human health.