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BODIPY 630/650 Acid
| Catalog Number | F01-0180 |
| Category | BODIPY |
| Molecular Formula | C23H17BF2N2O3S |
| Molecular Weight | 450.27 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 630/650 Acid is a fluorescent dye belonging to the BODIPY family, known for its boron-dipyrromethene core structure, which provides high photostability and intense fluorescence. It features an absorption maximum around 630 nm and emits at approximately 650 nm, making it suitable for red fluorescence imaging applications. The carboxylic acid functional group allows for easy conjugation to biomolecules, enabling its use in labeling proteins and nucleic acids for fluorescence microscopy and flow cytometry.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | EverFluor 630/650 Acid |
Product Specification
| Excitation | 494 |
| Emission | 520 |
Application
BODIPY 630/650 Acid is a fluorescent dye renowned for its stability and brightness in various applications. Here are some key applications of BODIPY 630/650 Acid:
Fluorescence Microscopy: BODIPY 630/650 Acid is widely used in fluorescence microscopy due to its exceptional photostability and high quantum yield. This dye provides sharp and bright images, making it ideal for live-cell imaging studies. Researchers can use it to track cellular processes such as protein localization and interaction with high sensitivity and precision.
Flow Cytometry: In flow cytometry, BODIPY 630/650 Acid serves as a reliable fluorescent label for analyzing cell populations. Its emission spectrum allows it to be distinguished from other commonly used dyes, enabling multiplexing in complex samples. This application is crucial for immunophenotyping, detecting fluorescently labeled antibodies or ligands that bind to specific cell surface markers.
Fluorescence Resonance Energy Transfer (FRET) Studies: BODIPY 630/650 Acid’s fluorescence properties make it suitable for FRET applications, which study molecular interactions and distances. By pairing it with suitable FRET partners, researchers can investigate conformational changes, protein-protein interactions, and nucleic acid dynamics in real-time. This provides invaluable insights into the molecular mechanisms underpinning biological functions.
Biochemical Assays: BODIPY 630/650 Acid is utilized in various biochemical assays to monitor enzyme activity, detect analytes, or measure protein concentrations. Its fluorescent properties make it a sensitive reporter for high-throughput screening assays in drug discovery. This application aids in uncovering novel compounds and understanding biochemical pathways relevant to human health.
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