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BODIPY
BODIPY 558/568 C3

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BODIPY 558/568 C3

Catalog Number	F01-0182
Category	BODIPY
Molecular Formula	C16H13BF2N2S
Molecular Weight	346.16

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Product Introduction

BODIPY 558/568 C3 is an orange-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. Carboxylic acids may also be used to form ester-linked conjugates or acid-halide products. It is non-ionic, and relatively insensitive to environment and pH changes.

Chemical Information
Product Specification
Application

Synonyms	EverFluor 558/568 C3
Appearance	Solid Powder

Excitation	494
Emission	520

BODIPY 558/568 C3 is a fluorescent dye known for its versatility in various biological and chemical applications. Here are some key applications of BODIPY 558/568 C3:

Fluorescence Microscopy: BODIPY 558/568 C3 is widely used in fluorescence microscopy for labeling and imaging biomolecules within cells. The dye’s bright fluorescence and photostability make it ideal for live-cell imaging and tracking cellular processes over time. Researchers can conjugate BODIPY 558/568 C3 to proteins, nucleic acids, or lipids to study their localization and dynamics in real-time.

Flow Cytometry: The unique spectral properties of BODIPY 558/568 C3 make it highly suitable for flow cytometry applications. It can be used to stain cells or cellular components, allowing for the analysis of cell populations based on fluorescence intensity. This is particularly useful in immunophenotyping, apoptosis studies, and cell cycle analysis.

FRET Studies: BODIPY 558/568 C3 is employed in Förster Resonance Energy Transfer (FRET) studies to investigate molecular interactions and distances. As an efficient FRET donor or acceptor, it can be paired with other compatible fluorophores to measure energy transfer events. This application is invaluable for studying protein-protein interactions and conformational changes in complex biological systems.

Chemical Sensing: BODIPY 558/568 C3 is used in chemical sensing and biosensor development due to its sensitivity to environmental changes. It can be incorporated into sensors to detect changes in pH, ions, or the presence of specific biomolecules. This application extends to environmental monitoring, clinical diagnostics, and high-throughput screening of chemical libraries.