![(E)-10-(4-(5,5-dimethyl-1,3,2-dioxaborinan-2-yl)phenyl)-3-(4-(dimethylamino)styryl)-5,5-difluoro-1,7,9-trimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide](https://resource.bocsci.com/structure/949108-72-9.gif)
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BODIPY 558/568 C3
| Catalog Number | F01-0182 |
| Category | BODIPY |
| Molecular Formula | C16H13BF2N2S |
| Molecular Weight | 346.16 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 558/568 C3 is a boron-dipyrromethene (BODIPY) dye recognized for its distinct photophysical properties, including a narrow emission spectrum and high fluorescence quantum yield. It features a conjugated aromatic system that facilitates efficient energy transfer, making it suitable for use as a fluorescence resonance energy transfer (FRET) component in complex biological assays. BODIPY 558/568 C3 is commonly used in fluorescence microscopy and flow cytometry applications due to its ability to conjugate with biomolecules, enabling precise localization and imaging of cellular structures.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | EverFluor 558/568 C3 |
| Appearance | Solid Powder |
Product Specification
| Excitation | 494 |
| Emission | 520 |
Application
BODIPY 558/568 C3 is a fluorescent dye known for its versatility in various biological and chemical applications. Here are some key applications of BODIPY 558/568 C3:
Fluorescence Microscopy: BODIPY 558/568 C3 is widely used in fluorescence microscopy for labeling and imaging biomolecules within cells. The dye’s bright fluorescence and photostability make it ideal for live-cell imaging and tracking cellular processes over time. Researchers can conjugate BODIPY 558/568 C3 to proteins, nucleic acids, or lipids to study their localization and dynamics in real-time.
Flow Cytometry: The unique spectral properties of BODIPY 558/568 C3 make it highly suitable for flow cytometry applications. It can be used to stain cells or cellular components, allowing for the analysis of cell populations based on fluorescence intensity. This is particularly useful in immunophenotyping, apoptosis studies, and cell cycle analysis.
FRET Studies: BODIPY 558/568 C3 is employed in Förster Resonance Energy Transfer (FRET) studies to investigate molecular interactions and distances. As an efficient FRET donor or acceptor, it can be paired with other compatible fluorophores to measure energy transfer events. This application is invaluable for studying protein-protein interactions and conformational changes in complex biological systems.
Chemical Sensing: BODIPY 558/568 C3 is used in chemical sensing and biosensor development due to its sensitivity to environmental changes. It can be incorporated into sensors to detect changes in pH, ions, or the presence of specific biomolecules. This application extends to environmental monitoring, clinical diagnostics, and high-throughput screening of chemical libraries.
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