![(E)-10-(4-(5,5-dimethyl-1,3,2-dioxaborinan-2-yl)phenyl)-3-(4-(dimethylamino)styryl)-5,5-difluoro-1,7,9-trimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide](https://resource.bocsci.com/structure/949108-72-9.gif)
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BODIPY 558/568 C12, SE
| Catalog Number | F01-0184 |
| Category | BODIPY |
| Molecular Formula | C29H34BF2N3O4S |
| Molecular Weight | 569.47 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 558/568 C12, SE is a member of the BODIPY fluorophore family, characterized by its boron-dipyrromethene core, which provides a robust and stable fluorescent signal. This compound features a succinimidyl ester reactive group, facilitating covalent conjugation with primary amine-containing biomolecules, enabling its use in protein labeling and cellular imaging applications. Exhibiting excitation and emission maxima in the orange-red spectral region, BODIPY 558/568 C12, SE is often utilized in fluorescence microscopy and flow cytometry for tracking lipid metabolism and visualizing membrane dynamics.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | EverFluor 558/568 C12, SE |
| Appearance | Solid Powder |
Product Specification
| Excitation | 533 |
| Emission | 549 |
Application
BODIPY 558/568 C12 SE is a fluorescent dye used extensively in bioscience research for its superior photostability and bright emission. Here are some key applications of BODIPY 558/568 C12 SE:
Live Cell Imaging: BODIPY 558/568 C12 SE is ideal for live cell imaging studies, allowing researchers to label cells and track their dynamics in real-time. Its photostability ensures prolonged observation periods without significant photobleaching. This dye helps in visualizing processes such as cell division, migration, and organelle trafficking.
Lipid Tracking: The lipophilic nature of BODIPY 558/568 C12 SE makes it suitable for staining lipids and studying lipid metabolism. Researchers can use this dye to track the incorporation of lipids into cellular membranes and their distribution within cells. This application is crucial in studying diseases related to lipid metabolism, such as obesity and cardiovascular disorders.
Flow Cytometry: BODIPY 558/568 C12 SE is frequently employed in flow cytometry for the detection and analysis of cell populations based on their fluorescence properties. It can be used to label specific cell surface markers or intracellular components, aiding in the identification of cell types and states. Its compatibility with other fluorophores allows for multiparametric analysis in complex cellular environments.
Protein Labeling: BODIPY 558/568 C12 SE can be conjugated to proteins for a variety of biochemical studies. It provides a means to visualize interactions between proteins, monitor protein localization, and study protein dynamics in both in vitro and in vivo settings. This application is essential in understanding protein function and interaction networks, leading to insights into cellular processes and disease mechanisms.
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