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BODIPY
BODIPY 530/550 C3

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BODIPY 530/550 C3

Catalog Number	F01-0181
Category	BODIPY
Molecular Formula	C24H19BF2N2O2
Molecular Weight	416.23

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Product Introduction

BODIPY 530/550 C3 is an orange-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. Carboxylic acids may also be used to form ester-linked conjugates or acid-halide products. It is non-ionic, and relatively insensitive to environment and pH changes.

Chemical Information
Product Specification
Application

Synonyms	EverFluor 530/550 C3; 4,4-Difluoro-5,7-diphenyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid
Appearance	Solid Powder

Excitation	494
Emission	520

BODIPY 530/550 C3, a fluorescent dye renowned for its exceptional photostability and vivid emission, finds extensive utility in diverse biological and chemical realms.

Fluorescence Microscopy: Widely employed in fluorescence microscopy, BODIPY 530/550 C3 stands out for its intense fluorescence and remarkable resistance to photobleaching, making it a preferred choice for prolonged imaging investigations. Scientists utilize this dye to label specific proteins or organelles, enabling the visualization and analysis of their spatial distribution and dynamic movement within living cells.

Flow Cytometry: In the realm of flow cytometry, BODIPY 530/550 C3 emerges as a valuable fluorescent marker for the analysis and sorting of cells based on distinctive properties. By conjugating this dye with antibodies or other biomolecules to target cells, researchers gain insights into cellular characteristics such as size, granularity, and protein expression. The dye’s distinct emission wavelengths facilitate multiplexing with other fluorochromes, enhancing the complexity of experimental designs.

FRET Applications: Within Förster Resonance Energy Transfer (FRET) studies, BODIPY 530/550 C3 plays a pivotal role as an energy donor or acceptor, unraveling molecular interactions with precision. Paired with compatible FRET partners, this dye aids in quantifying distances and conformational changes between biomolecules, shedding light on protein-protein interactions and underlying molecular mechanisms.

Chemical Sensing: Leveraged in the creation of cutting-edge chemical sensors, BODIPY 530/550 C3 enables the detection of diverse analytes such as ions or small molecules. Its fluorescence responsiveness to environmental alterations endows it with the ability to serve as a potent reporter in sensing assays. This application holds substantial significance in environmental surveillance, biosensing applications, and the development of diagnostic devices, offering unparalleled sensitivity and specificity for target identification.