BDP TR NHS ester | CAS 150152-65-1

BDP TR NHS ester is a reactive BODIPY-based fluorescent dye supplied as an N-hydroxysuccinimide (NHS) ester for efficient covalent labeling of primary amines. In fluorescence labeling workflows, its bright emission and amine-reactive chemistry enable the preparation of fluorescent protein and peptide conjugates, as well as dye-labeled biomolecules for imaging and assay development. The BDP TR scaffold is commonly used as a red-emitting fluorophore in microscopy and fluorescence readouts, where stable conjugation is required for downstream experiments.

1. Protein And Peptide Labeling

BDP TR NHS ester is widely used by protein biochemists and chemical biology teams to generate fluorescently labeled antibodies, enzymes, and affinity reagents through covalent attachment to lysine residues and N-terminal amines. Labeled proteins prepared with this NHS ester are commonly employed for fluorescence microscopy localization studies, fluorescence-based binding assays, and workflow development where consistent dye loading and stable conjugation are important. Researchers also use the resulting labeled peptides and proteins as fluorescent tracers in biomolecular interaction experiments and reagent characterization steps.

2. Fluorescence Microscopy Tracing

BDP TR NHS ester supports cellular and biomaterial imaging workflows where amine-containing targets need robust fluorescent tagging for localization and tracking. In microscopy applications, the dye-labeled biomolecules produced from this NHS ester are used to visualize binding to extracellular matrices, labeling of protein components on cell surfaces, and tracking of labeled macromolecules during incubation and wash steps. The red-emitting BDP TR fluorophore is often selected for multicolor experimental designs when spectral separation from common green channels is required, and the NHS ester chemistry helps ensure that labeling is covalent rather than relying on weak adsorption.

3. Flow Cytometry Conjugate Preparation

BDP TR NHS ester is used in flow cytometry reagent development to prepare fluorescent conjugates from amine-bearing biomolecules, including protein ligands and detection reagents. In typical workflows, researchers label targeting proteins or secondary reagents with the dye to create stable, covalently attached fluorescence reporters for cell staining experiments and fluorescence readouts. This chemistry is particularly useful when conjugate stability through fixation, washing, or repeated assay steps is a priority for consistent cytometry signals.

4. Fluorescent Bioassay Reagent Development

BDP TR NHS ester is frequently incorporated into fluorescence assay development to create labeled components that report binding, localization, or immobilization events. Assay developers use dye-labeled proteins and biomolecule conjugates to build fluorescence-based binding assays, imaging-compatible reagent sets, and fluorescent standards for instrument method development. Because NHS ester labeling targets primary amines, it is compatible with a broad range of amine-containing assay reagents, enabling rapid generation of fluorescent conjugates for screening and assay optimization in research laboratories.