BDP 630/650 X NHS ester | CAS 2213445-35-1

BDP 630/650 X NHS ester is a reactive fluorescent dye for covalent labeling of primary amines on proteins, peptides, and other amine-bearing biomolecules. As an NHS ester, it is commonly used to prepare fluorescent conjugates for fluorescence imaging and quantitative fluorescence workflows, with excitation/emission well matched to far-red detection channels. Researchers rely on its dye stability in labeling workflows to generate consistent conjugates for microscopy, assay development, and instrument-based readouts.

1. Protein And Antibody Labeling

BDP 630/650 X NHS ester is frequently used by protein chemistry and chemical biology groups to generate far-red fluorescently labeled proteins for downstream binding studies, imaging experiments, and fluorescence-based assays. The NHS ester reacts with lysine residues and N-termini to form stable amide linkages, enabling straightforward preparation of labeled antibodies, antibody fragments, enzymes, and affinity reagents. In practice, teams use the resulting conjugates to track biomolecule distribution, quantify binding in plate-based formats, or visualize labeled targets in fluorescence microscopy workflows that benefit from far-red spectral separation.

2. Fluorescence Microscopy Tracing

BDP 630/650 X NHS ester supports cellular and biomaterial imaging workflows where far-red fluorescence is advantageous for reducing spectral crosstalk and improving signal discrimination. Lab groups use the dye to label amine-containing components such as extracellular matrix proteins, cell-surface proteins, or probe-bearing carriers, then visualize localization patterns by fluorescence microscopy. Because the labeling is covalent, it is often selected for multi-step staining or wash-intensive imaging protocols where retention of fluorescence signal on the labeled biomolecule is important for consistent imaging across samples.

3. Flow Cytometry Labeling Reagents

BDP 630/650 X NHS ester is used to prepare fluorescent conjugates for flow cytometry and related cell analysis workflows when a far-red dye channel is desired. Immunology and cell biology laboratories commonly label amine-bearing proteins or targeting ligands to generate fluorescent reagents that can be incubated with cells and analyzed by flow cytometry. Covalent dye attachment helps maintain conjugate integrity during staining and washing steps, supporting reproducible staining intensity across experimental replicates and enabling quantitative comparisons of labeled biomolecule association with cell populations.

4. Fluorescent Conjugate Standards

BDP 630/650 X NHS ester is also employed in reagent development and assay standardization workflows to create far-red fluorescent conjugates for calibration, method validation, and instrument setup. Researchers and assay developers use the dye-labeled biomolecules as fluorescence references in plate readers and imaging systems, helping establish consistent measurement conditions across experiments. This application is particularly useful when far-red detection is required to align with existing optical filters or to support multiplex panels that separate the BDP 630/650 signal from other fluorophores.