6,8-difluoro-4-methylumbelliferyl phosphate | 214491-43-7

6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP), a widely used fluorogenic substrate in bioscience research, has diverse applications. Here are four key applications of DiFMUP:

Enzyme Activity Assays: Serving as a substrate for phosphatase enzyme activity assays, DiFMUP undergoes dephosphorylation by phosphatases, resulting in the release of a fluorescent product for easy detection and quantification. This unique property makes DiFMUP an exceptional tool for measuring enzyme kinetics and screening potential inhibitors in drug discovery, offering insights into the intricate world of enzymatic reactions.

Cell Signaling Studies: In the realm of cell signaling pathways, DiFMUP finds its place by aiding in the quantification of phosphatase activity, particularly vital in understanding signal transduction processes under diverse conditions. By decoding how these enzymes function, researchers gain profound insights into cellular responses to various stimuli, unraveling potential therapeutic targets amidst the complex web of intracellular communication.

Cancer Research: Embracing the realm of cancer research, DiFMUP emerges as a valuable tool for studying phosphatase activity in tumor cells. With variations in phosphatase activity serving as potential indicators of oncogenic processes, DiFMUP-based assays offer a window into diagnosing and characterizing different cancer types.

High-Throughput Screening: Equipped for high-throughput screening (HTS) assays in pharmaceutical research, DiFMUP’s fluorogenic properties pave the way for rapid and sensitive detection of enzyme activity within vast compound libraries. This streamlined approach accelerates the identification of new drug candidates, propelling the drug development process forward with agility and precision, shaping the future of pharmaceutical innovation.