5,5'-Dibromo BAPTA | CAS 73630-11-2

Catalog Number	A14-0127
Category	Calcium, Chloride and Other indicators
Molecular Formula	C22H22Br2N2O10
Molecular Weight	634.23

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Product Introduction

5,5'-Dibromo BAPTA is a calcium-sensitive fluorescent dye that belongs to the BAPTA family of chelators, characterized by its dibromo-substituted aromatic rings which enhance its metal ion affinity. The compound exhibits unique spectral properties, allowing it to participate in calcium imaging applications by undergoing fluorescence changes upon calcium binding, making it suitable for tracking intracellular calcium dynamics. It is commonly used in fluorescence microscopy and biosensing assays where precise calcium ion detection is crucial for understanding cellular signaling pathways.

Chemical Information
Application

Chemical Information

SMILES	C1=CC(=C(C=C1Br)OCCOC2=C(C=CC(=C2)Br)N(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O
InChI	InChI=1S/C22H22Br2N2O10/c23-13-1-3-15(25(9-19(27)28)10-20(29)30)17(7-13)35-5-6-36-18-8-14(24)2-4-16(18)26(11-21(31)32)12-22(33)34/h1-4,7-8H,5-6,9-12H2,(H,27,28)(H,29,30)(H,31,32)(H,33,34)
InChIKey	KCTRRYZOCJDOTC-UHFFFAOYSA-N
Appearance	Solid Powder

Application

Calcium Ion Sensing: 5,5’-Dibromo BAPTA is primarily used as a calcium ion chelator in biological and chemical research. Its high affinity for calcium ions allows for precise measurements of intracellular calcium levels, crucial in understanding various physiological processes such as muscle contraction, neurotransmitter release, and cell signaling. Researchers often utilize 5,5’-Dibromo BAPTA in fluorescence microscopy to visualize calcium dynamics in live cells, enabling them to explore the role of calcium in signaling pathways. This application is fundamental in studies related to neurobiology and cardiology, where calcium signaling plays a pivotal role.

Pharmacological Research: In pharmacological studies, 5,5’-Dibromo BAPTA serves as a tool for dissecting calcium-dependent mechanisms of drug action. By chelating calcium, it helps in elucidating the pathways through which various drugs exert their effects, particularly in the context of calcium-mediated signaling. This application is vital in the development of therapeutics targeting diseases associated with calcium dysregulation, such as hypertension and arrhythmias. Researchers can manipulate calcium levels using 5,5’-Dibromo BAPTA to assess drug efficacy and safety, contributing to the advancement of new pharmacological agents.

Investigating Apoptosis: Another significant application of 5,5’-Dibromo BAPTA is in apoptosis research. Calcium ions play a crucial role in the regulation of programmed cell death, and 5,5’-Dibromo BAPTA’s ability to sequester calcium allows scientists to investigate the mechanisms underlying apoptosis. By altering calcium levels in cells, researchers can determine the contribution of calcium to apoptotic signaling pathways, providing insights into cancer biology and potential therapeutic targets. This application is particularly relevant in developing anti-cancer therapies that exploit calcium signaling pathways to induce cell death in malignant cells.

Neuroscience Studies: In the field of neuroscience, 5,5’-Dibromo BAPTA is extensively used to study synaptic transmission and plasticity. Calcium influx through channels is crucial for neurotransmitter release, and by using 5,5’-Dibromo BAPTA, researchers can examine how alterations in calcium levels affect synaptic strength and plasticity. This application is essential for understanding learning and memory mechanisms, as well as the pathophysiology of neurodegenerative diseases. By manipulating calcium signaling with 5,5’-Dibromo BAPTA, scientists can explore the intricacies of neuronal communication and the effects of various interventions on synaptic function.