4,4-Difluoro-8(3',4'-dihydroxylphenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene | CAS 1150586-09-6

4,4-Difluoro-8(3',4'-dihydroxylphenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene is a fluorescent dye widely used in scientific research due to its unique photophysical properties.

Fluorescence Microscopy: This compound is extensively used in fluorescence microscopy to stain biological specimens, allowing researchers to visualize cellular components with high specificity and contrast. Its strong fluorescence signal and stability under various conditions make it ideal for long-term imaging studies. This enables detailed observation of dynamic biological processes at the cellular level.

Flow Cytometry: In flow cytometry, 4,4-Difluoro-8(3',4'-dihydroxylphenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene serves as a fluorescent tag to label cells and analyze their properties individually. Its distinctive fluorescence helps in accurately quantifying and characterizing cell populations based on specific markers. This application is crucial for diagnostics, particularly in immunophenotyping and cancer research.

Fluorescence Resonance Energy Transfer (FRET): This dye is employed in FRET-based experiments to study protein-protein interactions and conformational changes in real-time. By acting as a donor or acceptor molecule, it facilitates the transfer of energy between closely positioned biomolecules. This provides insights into the molecular mechanisms underpinning cellular functions and interactions.

Biomedical Imaging: The compound can be used in biomedical imaging to highlight structures and study physiological processes in living organisms. Its capacity to penetrate tissues and emit bright fluorescence aids in non-invasive imaging techniques. This utility extends to tracking drug delivery, assessing tissue distribution, and monitoring metabolic activities in vivo.