3,7-bis((E)-2-(anthracen-9-yl)vinyl)-5,5-difluoro-1,9-dimethyl-10-(p-tolyl)-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide | CAS 1432580-28-3

3,7-bis((E)-2-(anthracen-9-yl)vinyl)-5,5-difluoro-1,9-dimethyl-10-(p-tolyl)-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is a specialized fluorescent dye framework designed for optical signal generation in fluorescence-based research workflows. Its extended conjugation and anthracene-containing motifs support strong light-harvesting and emission, making it useful when robust fluorescence readout is required for imaging, labeling, and assay development. The cationic boron-dipyrromethene-like scaffold also supports incorporation into labeling strategies where controlled dye-to-biomolecule conjugation and consistent optical performance are important.

1. Fluorescence Labeling Probes

3,7-bis((E)-2-(anthracen-9-yl)vinyl)-5,5-difluoro-1,9-dimethyl-10-(p-tolyl)-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is used by chemical biology groups to build fluorescent labeling reagents for microscopy and fluorescence imaging experiments. Researchers commonly incorporate this dye into conjugates with biomolecular carriers (for example, via established dye-bioconjugation chemistries in their platform) to visualize labeled targets in fixed-sample studies and assay formats where bright, stable fluorescence is required. The dye's highly conjugated structure supports strong optical output, which is valuable for constructing imaging reagents for localization studies and fluorescence-based tracking of labeled constructs.

2. Fluorescence Microscopy Imaging

3,7-bis((E)-2-(anthracen-9-yl)vinyl)-5,5-difluoro-1,9-dimethyl-10-(p-tolyl)-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide supports cellular and materials microscopy workflows where fluorescence intensity and spectral behavior are critical for clear visualization. Imaging teams use this dye to stain or label prepared specimens and to generate high-contrast fluorescence signals for confocal or widefield microscopy experiments. Because the dye is structurally designed for strong emission, it is often selected when researchers need a fluorescent reporter that performs reliably as part of a multicomponent imaging scheme, including dye-combination studies and fluorescence readouts from labeled biomaterials.

3. Fluorescence Bioassay Readouts

3,7-bis((E)-2-(anthracen-9-yl)vinyl)-5,5-difluoro-1,9-dimethyl-10-(p-tolyl)-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is applied in fluorescence assay development where a strong dye signal improves the practicality of optical measurements. Assay developers use this fluorophore as a reporter component in plate-based fluorescence workflows, including binding/interaction readouts and endpoint fluorescence assays for labeled reagents. Its extended conjugation and anthracene-containing architecture support bright emission, which can be leveraged when optimizing assay protocols for consistent fluorescence detection on standard fluorescence plate readers and imaging systems.

4. FRET Pair Construction

3,7-bis((E)-2-(anthracen-9-yl)vinyl)-5,5-difluoro-1,9-dimethyl-10-(p-tolyl)-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is also used by fluorescence technology teams to design Förster resonance energy transfer (FRET) architectures for distance- or assembly-dependent signal reporting. Researchers select this dye as a donor or acceptor component in FRET pair construction when they want an intense fluorescence contributor that can be incorporated into labeled constructs. In such systems, the dye's optical output helps define the measurable fluorescence response upon energy transfer, supporting the development of fluorescence-based molecular imaging reagents and assay tools that translate molecular proximity into a readout.