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3,3'-Diethylthiatricarbocyanine iodide | 3071-70-3
| Catalog Number | A17-0070 |
| Category | Laser Dyes |
| Molecular Formula | C25H25IN2S2 |
| Molecular Weight | 544.51 |
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Product Introduction
3,3'-Diethylthiatricarbocyanine iodide is an extensively employed fluorescent dye in the biomedical sector. It serves as a pivotal stain for live cell imaging and flow cytometry trials due to its binding capacity to mitochondria. By facilitating the visualization and evaluation of mitochondrial morphology and functionality, this dye exhibits unparalleled prowess in investigating cellular metabolism and dynamics. Noteworthy attributes encompass its superior photostability, rendering it indispensable in comprehensive mitochondrial research endeavors.
Chemical Information |
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| Synonyms | Benzothiazolium, 3-ethyl-2-[7-(3-ethyl-2(3H)-benzothiazolylidene)-1,3,5-heptatrien-1-yl]-, iodide (1:1); 3-ethyl-2-[7-(3-ethyl-2(3H)-benzothiazolylidene)-1,3,5-heptatrienyl]-benzothiazolium iodide; DTTC Iodide |
| Canonical SMILES | CCN1C2=CC=CC=C2SC1=CC=CC=CC=CC3=[N+](C4=CC=CC=C4S3)CC.[I-] |
| InChI | InChI=1S/C25H25N2S2.HI/c1-3-26-20-14-10-12-16-22(20)28-24(26)18-8-6-5-7-9-19-25-27(4-2)21-15-11-13-17-23(21)29-25;/h5-19H,3-4H2,1-2H3;1H/q+1;/p-1 |
| InChI Key | OYVFJKVYVDYPFV-UHFFFAOYSA-M |
- Application
3,3’-Diethylthiatricarbocyanine iodide is a synthetic dye with multiple applications in bioscience and medical research. Here are some key applications of 3,3’-Diethylthiatricarbocyanine iodide:
Fluorescence Microscopy: 3,3’-Diethylthiatricarbocyanine iodide is commonly used as a fluorescent dye in microscopy to label cellular components. This compound binds to specific cellular structures, emitting fluorescence under particular wavelengths of light. This allows scientists to visualize intricate details within cells, aiding in various research and diagnostic applications.
Flow Cytometry: In flow cytometry, 3,3’-Diethylthiatricarbocyanine iodide serves as a vital staining dye to label and identify different cell populations. By tagging cells with this fluorescent dye, researchers can analyze cell size, complexity, and protein expression on a single-cell level. This application is crucial in immunology, cancer research, and cell biology for identifying and sorting diverse cell types.
Medical Imaging: The dye is also used in medical imaging to enhance the contrast of specific tissues or cellular components. When administered to patients or used in cell cultures, it helps highlight areas of interest, improving the accuracy and detail of imaging studies. This application is particularly useful in diagnosing diseases and monitoring the efficacy of treatments.
Cell Viability Assays: 3,3’-Diethylthiatricarbocyanine iodide can be utilized in cell viability assays to assess the health and proliferation of cells. The dye penetrates living cells where its fluorescence can be measured to determine cell viability and metabolic activity. This is essential for drug screening, cytotoxicity testing, and evaluating cellular responses to various treatments.
Applications of Fluorescent Probes & Dyes
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