10-(3-amino-4-hydroxyphenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide | CAS 1181844-41-6

10-(3-amino-4-hydroxyphenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is a boron-dipyrromethene (BODIPY)-type fluorescent dye engineered for conjugation workflows, combining a highly fluorescent core with an aniline and phenolic functionality that can be leveraged for covalent attachment to biomolecules and materials. Its strong fluorescence enables sensitive visualization in labeling and imaging pipelines, while the reactive aromatic groups support downstream derivatization into fluorescent conjugates for chemical biology and assay development.

1. Biomolecule Labeling

10-(3-amino-4-hydroxyphenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is used by chemical biology and protein chemistry groups to prepare fluorescently labeled antibodies, peptides, and other affinity reagents where a BODIPY-like scaffold provides robust signal for microscopy and fluorescence readouts. Researchers typically incorporate the dye into conjugation strategies to generate stable dye-biomolecule conjugates for tracking binding, studying biomolecular interactions, and building labeled standards for assay calibration.

2. Fluorescence Microscopy

10-(3-amino-4-hydroxyphenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide supports fluorescence microscopy applications where covalent attachment to targeting ligands or surface coatings is preferred over non-specific adsorption. In cell imaging and materials imaging workflows, the dye is commonly used as a bright reporter to visualize labeled biomolecules on substrates, map conjugate distribution, and quantify fluorescence intensity changes in imaging-based assays using standard epifluorescence and confocal instrumentation.

3. Flow Cytometry Assay Reagents

10-(3-amino-4-hydroxyphenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is employed in flow cytometry reagent development to create fluorescent conjugates for population-level analysis of labeled biomolecules and binding events. Laboratories use dye-biomolecule constructs to generate stable staining reagents for fluorescence-based quantification, including assay development where reproducible labeling chemistry and consistent fluorescence output are important for gating, normalization, and comparative studies across experimental conditions.

4. Fluorescent Probe Construction

10-(3-amino-4-hydroxyphenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide is incorporated into fluorescent probe construction for molecular imaging reagent development, particularly when a conjugatable fluorophore is required to attach recognition elements or reporter modules. The dye's aromatic amine and phenolic functionality are leveraged to build modular probe conjugates that can be further functionalized for labeling strategies in biochemical assays, enabling fluorescence-based monitoring of labeled targets and reporter localization in experimental platforms that rely on covalent probe attachment.