1,5,7-Trimethyl-3-(1-pyrenylethenyl)-8-(4-iodophenyl)-4,4-difluoroboradiazasindacene | CAS 1256917-87-9

1,5,7-Trimethyl-3-(1-pyrenylethenyl)-8-(4-iodophenyl)-4,4-difluoroboradiazasindacene is a boron-difluoride difluoroboradiazasindacene (BODIPY) fluorophore engineered for strong fluorescence and extended conjugation, with a pyrene-ethenyl substituent that supports high photophysical performance in fluorescence labeling workflows. The presence of an aryl iodide enables downstream derivatization for conjugate construction, making this dye useful when researchers need a bright imaging label that can be incorporated into larger fluorescent probes or material-bound reporters.

1. Fluorescence Labeling Conjugates

1,5,7-Trimethyl-3-(1-pyrenylethenyl)-8-(4-iodophenyl)-4,4-difluoroboradiazasindacene is used by chemical biology and medicinal chemistry teams to build fluorescent conjugates for tracking biomolecules and macromolecular assemblies. Researchers commonly incorporate this dye into labeling reagents for proteins, peptides, and affinity reagents where a robust fluorophore is required for microscopy readouts or fluorescence-based quantification. The aryl iodide handle supports modular synthetic attachment to targeting ligands or linkers during probe construction, enabling consistent dye loading on custom conjugate architectures.

2. Fluorescence Microscopy Imaging

1,5,7-Trimethyl-3-(1-pyrenylethenyl)-8-(4-iodophenyl)-4,4-difluoroboradiazasindacene supports fluorescence microscopy workflows where bright emission and conjugation-driven optical performance improve visualization of labeled targets. Imaging teams use this fluorophore in fixed-sample studies and in fluorescence imaging experiments that rely on stable, high-contrast labeling of tagged biomolecular structures. The extended aromatic character and BODIPY core make it a practical choice for constructing imaging reagents that can be optimized for specific excitation/emission filter sets used in standard epifluorescence and confocal microscopy.

3. Fluorescent Probe Development

1,5,7-Trimethyl-3-(1-pyrenylethenyl)-8-(4-iodophenyl)-4,4-difluoroboradiazasindacene is frequently selected for fluorescent probe development where a modular dye scaffold is needed to generate structure-specific reporters. Probe developers use this compound as a core fluorophore for assembling multi-component sensing constructs, such as conjugates that include recognition motifs, affinity handles, or reporter linkers for imaging and assay readouts. The iodinated aryl substituent provides a convenient functional handle for incorporating the dye into larger probe designs, supporting iterative optimization of labeling density, linker length, and conjugate format for research-grade fluorescence studies.

4. Materials and Surface Fluorescent Tagging

1,5,7-Trimethyl-3-(1-pyrenylethenyl)-8-(4-iodophenyl)-4,4-difluoroboradiazasindacene is applied in biomaterials and surface science to create fluorescently tagged polymers, coatings, and engineered interfaces used for material characterization. Researchers use the dye to visualize attachment, distribution, and stability of fluorescently labeled materials in imaging-based studies, including mapping of labeled domains on surfaces and tracking of dye-containing layers in materials workflows. The aryl iodide functionality supports derivatization strategies that enable incorporation of the fluorophore into material-bound constructs used for laboratory-scale fluorescence characterization.