Tetramethylrhodamine methyl ester perchlorate
Catelog Number: A16-0053
Category: Cell and Orgenella Stains
Description
Chemical Information
Product Specification
Additional Information
Tetramethylrhodamine methyl ester (TMRM) perchlorate is a cell-permeant red fluorescent probe commonly used to monitor membrane potential of mitochondria using live cell fluorescence microscopy and flow cytometry. TMRM is a lipophilic cation accumulated by mitochondria and exhibits a red shift in the emission of 575 nm. However, when the membrane is depolarized, as in apoptosis, TMRM is not accumulated in the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.
| Catelog Number: | A16-0053 |
| Product Name: | Tetramethylrhodamine methyl ester perchlorate |
| CAS Number: | 115532-50-8 |
| Molecular Formula: | C25H25N2O3·ClO4 |
| Molecular Weight: | 500.9 |
| Purity: | ≥98% |
| Synonyms: | TMRM Perchlorate; TMR methyl ester perchlorate; 3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium perchlorate |
| Appearance: | Solid Powder |
| Excitation Maximum: | 515, 555 |
| Storage Conditions: | Store at -20°C |
| InChI: | InChI=1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1 |
| InChIKey: | PFYWPQMAWCYNGW-UHFFFAOYSA-M |
| CanonicalSMILES: | CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=CC=CC=C4C(=O)OC.[O-]Cl(=O)(=O)=O |
