Tetramethylrhodamine methyl ester perchlorate

Catelog Number: A16-0053

Category: Cell and Orgenella Stains


Tetramethylrhodamine methyl ester (TMRM) perchlorate is a cell-permeant red fluorescent probe commonly used to monitor membrane potential of mitochondria using live cell fluorescence microscopy and flow cytometry. TMRM is a lipophilic cation accumulated by mitochondria and exhibits a red shift in the emission of 575 nm. However, when the membrane is depolarized, as in apoptosis, TMRM is not accumulated in the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.

Chemical Information
Catelog Number: A16-0053
Product Name: Tetramethylrhodamine methyl ester perchlorate
CAS Number: 115532-50-8
Molecular Formula: C25H25N2O3·ClO4
Molecular Weight: 500.9
Purity: ≥98%
Synonyms: TMRM Perchlorate; TMR methyl ester perchlorate; 3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium perchlorate
Product Specification
Appearance: Solid Powder
Excitation Maximum: 515, 555
Storage Conditions: Store at -20°C
Additional Information
InChI: InChI=1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1
CanonicalSMILES: CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=CC=CC=C4C(=O)OC.[O-]Cl(=O)(=O)=O

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