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Resazurin sodium salt | CAS 62758-13-8
| Catalog Number | A16-0029 |
| Category | Cell Proliferation Tracer Fluorescent Probes |
| Molecular Formula | C12H6NNaO4 |
| Molecular Weight | 251.2 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A16-0029 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Resazurin is a blue non-fluorescent dye that is used as an oxidation-reduction indicator in cell viability assays. Resazurin generates red fluorescence when reduced to resorufin.
Chemical Information
Product Specification
Application
QC Data
| Related CAS | 550-82-3 (free base) |
| Synonyms | 7-hydroxy-3H-phenoxazin-3-one-10-oxide monosodium salt |
| Canonical SMILES | C1=CC2=C(C=C1[O-])OC3=CC(=O)C=CC3=[N+]2[O-].[Na+] |
| InChI | InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1 |
| InChIKey | IVGPGQSSDLDOLH-UHFFFAOYSA-M |
| Appearance | Solid Powder |
| Excitation | 530-540 nm |
| Emission | 585-595 nm |
| Storage | Store at -20°C |
Resazurin sodium salt, a versatile redox indicator, finds wide-ranging applications in scientific research. Here are four key applications of Resazurin sodium salt：
Cell Viability Assays: Widely employed in cell viability and cytotoxicity assays, Resazurin sodium salt stands out for its unique ability to gauge metabolic activity in cells. Viable cells catalyze the reduction of resazurin to resorufin, emitting a fluorescent signal. This characteristic enables researchers to quantitatively assess cell proliferation, viability, and the cytotoxic effects of compounds in a precise and dependable manner.
Microbial Testing: A pivotal tool in microbiology, resazurin sodium salt functions as a reliable indicator for bacterial and yeast proliferation. When introduced into culture media, the reduction of resazurin to resorufin serves as a telltale sign of microbial growth. This technique is commonly utilized in antibiotic susceptibility tests and the quality control of microbiological processes.
Environmental Toxicology: In the realm of environmental studies, resazurin sodium salt plays a crucial role in evaluating the toxicity of pollutants on aquatic organisms. By subjecting cultured cells or microorganisms to environmental samples containing contaminants, the reduction of resazurin offers valuable insights into the detrimental effects of pollutants. This aids in the continuous monitoring of environmental well-being and the assessment of pollutant impacts.
Biofilm Formation Studies: Researchers turn to resazurin sodium salt to delve into the intricacies of biofilm formation by diverse microbial species. By examining the metabolic activity within biofilms, this compound serves as a key tool in unraveling the dynamics of biofilm structures. This application holds particular significance in medical research, contributing to the development of effective strategies for managing biofilm-related infections.
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