SynaptoRedTM C2 | CAS 162112-35-8

SynaptoRedTM C2 is a red-emitting fluorescent dye designed for synaptic vesicle and presynaptic terminal labeling in fluorescence microscopy workflows. Its membrane-associated staining behavior supports visualization of neuronal structures involved in neurotransmitter storage and release, making it useful for cell biology studies focused on synapse morphology and trafficking. Researchers typically pair SynaptoRedTM C2 with standard fluorescence imaging systems using appropriate filter sets for red emission to map presynaptic organization in fixed or prepared neuronal samples.

1. Presynaptic Terminal Labeling

SynaptoRedTM C2 is used by neuroscience and cell biology labs to stain presynaptic terminals and visualize synaptic vesicle-rich regions in cultured neurons and synapse-enriched preparations. In microscopy workflows, the dye provides a practical red fluorescence readout for comparing synaptic density and spatial organization across experimental conditions such as synapse maturation, stimulation paradigms, or perturbations affecting vesicle trafficking. Imaging datasets commonly support quantitative analyses of puncta density, cluster size, and colocalization with complementary synaptic markers labeled in other spectral channels.

2. Synapse Morphology Imaging

SynaptoRedTM C2 supports fluorescence imaging of synapse morphology in studies where researchers need consistent labeling of presynaptic structures across multiwell or multi-sample experiments. The red fluorescence signal is frequently leveraged for longitudinal comparisons in fixed timepoint studies, enabling visualization of changes in synaptic punctate patterns that accompany neuronal development or experimental manipulation. This application is commonly adopted in labs performing high-content microscopy screening of chemical biology tools, imaging-based phenotyping of synaptic alterations, and structural characterization of synaptic connectivity proxies.

3. Co-Localization With Markers

SynaptoRedTM C2 is frequently incorporated into multiplex staining strategies to assess colocalization between presynaptic vesicle-associated regions and other proteins, cytoskeletal components, or organelle markers. Researchers use the dye as a presynaptic reference channel when designing multicolor imaging panels, planning excitation/emission separation so that red puncta can be compared with independently labeled targets. This approach is especially valuable for mapping synaptic subdomains and for interpreting how experimental treatments shift the spatial relationship between vesicle-rich compartments and partner markers.

4. Neuronal Cell Biology Assays

SynaptoRedTM C2 is used in fluorescence-based neuronal assays where synaptic labeling serves as a structural endpoint for bioassay development and mechanistic cell biology studies. In practice, the dye is applied in imaging workflows that quantify synaptic staining intensity or puncta metrics to evaluate cellular responses to reagents that modulate synaptic function or vesicle dynamics. Teams building assay-ready protocols for microscopy and image analysis often select SynaptoRedTM C2 because it provides a straightforward fluorescent readout tied to presynaptic organization, supporting reproducible comparisons across experimental batches.