Sodium 4-[2-[(1E,3Z,5E,7E)-4-Carboxy-7-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]hepta-1,3,5-trien-1-yl]-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl]butane-1-sulfonate

Sodium 4-[2-[(1E,3Z,5E,7E)-4-Carboxy-7-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]hepta-1,3,5-trien-1-yl]-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl]butane-1-sulfonate is a highly sulfonated, water-soluble fluorescent dye designed for strong visible/near-visible absorption and bright emission, with multiple anionic sulfonate groups that support aqueous labeling workflows. Its conjugated polymethine framework and cationic/zwitterionic dye character make it useful for fluorescence-based imaging and quantitative staining where robust optical contrast is required. The presence of a carboxyl group also supports incorporation into labeling strategies and conjugate construction for microscopy and assay development.

1. Fluorescence Microscopy Labeling

Sodium 4-[2-[(1E,3Z,5E,7E)-4-Carboxy-7-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]hepta-1,3,5-trien-1-yl]-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl]butane-1-sulfonate is used by cell biology and materials imaging groups as a fluorescent labeling reagent for visualizing biomolecule conjugates and dye-tagged constructs under fluorescence microscopes. The dye's sulfonate-rich structure helps maintain water compatibility in staining buffers, supporting consistent signal during washes and multi-step workflows. Researchers commonly incorporate it into labeled probes or dye-conjugated biomaterials to track distribution, uptake, or surface association in fixed-cell studies, biointerface experiments, and fluorescence imaging of labeled polymers and nanoparticles.

2. Flow Cytometry Fluorescent Tracing

Sodium 4-[2-[(1E,3Z,5E,7E)-4-Carboxy-7-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]hepta-1,3,5-trien-1-yl]-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl]butane-1-sulfonate supports fluorescence-based quantification workflows where labeled populations need to be distinguished by optical intensity. In flow cytometry and fluorescence-activated sorting development, the dye is frequently used to generate stable fluorescent tags for conjugates that can be detected in aqueous assay buffers. Its high ionic character helps reduce aggregation tendencies in many labeling contexts, which is valuable when preparing reproducible staining reagents for instrument-based readouts of labeled biomolecules, dye-tagged affinity reagents, or fluorescently labeled material components.

3. Fluorescence Bioassay Signal Reporter

Sodium 4-[2-[(1E,3Z,5E,7E)-4-Carboxy-7-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]hepta-1,3,5-trien-1-yl]-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl]butane-1-sulfonate is applied as a fluorescent reporter dye in fluorescence assay development where conjugate formation yields a measurable optical signal in microplate and cuvette formats. Assay developers use it to label assay components such as binding reagents, tracer molecules, or dye-tagged standards to enable endpoint or kinetic fluorescence readouts. The dye's conjugated chromophore and water solubility profile support consistent fluorescence measurements across typical laboratory buffer conditions, making it useful for method development in screening, binding studies, and fluorescence-based characterization of labeled reagents.

4. Dye-Conjugate Construction For Probes

Sodium 4-[2-[(1E,3Z,5E,7E)-4-Carboxy-7-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]hepta-1,3,5-trien-1-yl]-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl]butane-1-sulfonate is frequently selected as a fluorophore building block for constructing dye conjugates used in molecular imaging reagent development and fluorescence probe engineering. Researchers leverage its functional group content, including the carboxyl moiety, to enable attachment to targeting vectors, affinity ligands, or scaffold materials during probe assembly workflows. In these applications, the dye's sulfonated framework supports aqueous handling and helps maintain fluorescence performance in conjugate formulations used for imaging and assay readouts, including labeled biomolecule tracers and fluorescently tagged biomaterial components.