
NHS-Ac-N3 | CAS 824426-32-6
| Catalog Number | R14-0325 |
| Category | Azides |
| Molecular Formula | C6H6N4O4 |
| Molecular Weight | 198.13 |
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Product Introduction
NHS-Ac-N3 is a bioorthogonal conjugation building block that provides a bioorthogonal click handle for modular click-chemistry workflows. The molecule can be used to introduce a click-reactive group onto an antibody, peptide, linker intermediate, or payload-containing component, depending on the chemistry of its second terminal functionality. Its additional reactive group is not clearly defined from product name; verify structure before assigning antibody conjugation chemistry. Conjugation can be performed using spaac with dbco/bcn-functionalized partner or cuaac with terminal alkyne partner, depending the complementary handle. The sequence and orientation of the coupling steps should be selected according to the functional groups carried by the two conjugation partners.
Chemical Information
Product Specification
Application
QC Data
Chemical Information
| Synonyms | Azidoacetic acid NHS ester;(2,5-Dioxopyrrolidin-1-yl) 2-azidoacetate; |
| Purity | ≥98% |
| IUPAC Name | (2,5-dioxopyrrolidin-1-yl) 2-azidoacetate |
| SMILES | C1CC(=O)N(C1=O)OC(=O)CN=[N+]=[N-] |
| InChI | InChI=1S/C6H6N4O4/c7-9-8-3-6(13)14-10-4(11)1-2-5(10)12/h1-3H2 |
| InChIKey | FENNDBOWHRZLTQ-UHFFFAOYSA-N |
| Appearance | White crystalline powder |
Product Specification
| Storage | Store at-20 °C |
Application
NHS-Ac-N3, a versatile chemical compound utilized in diverse bioconjugation and labeling applications, showcases its adaptability across various scientific endeavors. Here are four key applications of NHS-Ac-N3:
Protein Labeling: A common practice in the realm of protein research, NHS-Ac-N3 serves as a pivotal tool for labeling proteins with azide groups, enabling subsequent modifications through click chemistry. This strategy allows researchers to delve into protein interactions and cellular localization, shedding light on the intricacies of protein function and dynamics within biological systems.
Drug Delivery System Development: At the forefront of pharmaceutical innovation, NHS-Ac-N3 plays a critical role in crafting targeted drug delivery systems. By conjugating drug molecules or carriers with azide groups, researchers can attach specific targeting ligands via click chemistry, enhancing drug efficacy and minimizing off-target effects.
Biomolecule Synthesis: Empowering researchers in the synthesis of complex biomolecules, NHS-Ac-N3 enables site-specific modifications through the incorporation of reactive azide groups into nucleic acids, peptides, and other biomolecules. This level of precision offers unparalleled control over molecular assembly, fostering the engineering of novel biomolecular structures with targeted functionalities.
Bioconjugation: A cornerstone in the field of diagnostics and therapeutics, NHS-Ac-N3 emerges as an indispensable reagent for bioconjugation techniques. By facilitating the attachment of various biomolecules to surfaces or nanoparticles, this compound enhances detection sensitivity and therapeutic properties. The resulting bioconjugates play a pivotal role in the development of cutting-edge diagnostic assays and targeted therapeutic interventions, driving forward the frontier of biotechnological advancements.
QC Data
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