Lectin from Phytolacca americana-ATTO 647N conjugate

Lectin from Phytolacca americana-ATTO 647N conjugate is a fluorescent lectin conjugate designed for carbohydrate-dependent labeling in glycobiology workflows. The ATTO 647N fluorophore provides strong far-red emission for imaging experiments where reduced spectral overlap with common green fluorophores is advantageous. Researchers use this reagent to visualize lectin-binding patterns on cells, extracellular matrices, and glycan-decorated biomaterials, supporting microscopy-based localization studies and fluorescence readouts in assay development.

1. Glycan Binding Microscopy

Lectin from Phytolacca americana-ATTO 647N conjugate is used to map carbohydrate-dependent binding on cell surfaces and in fixed-cell preparations for fluorescence microscopy. Lab groups applying glycobiology and cell-surface characterization workflows rely on the far-red ATTO 647N signal to visualize lectin binding patterns while minimizing interference from typical green-channel stains. This conjugate is commonly incorporated into staining panels to compare glycan presentation across experimental conditions such as lectin competition, enzymatic glycan remodeling, or glycoprotein expression changes.

2. Flow Cytometry Lectin Labeling

Lectin from Phytolacca americana-ATTO 647N conjugate supports flow cytometry workflows that quantify lectin binding to cell populations. Immunophenotyping and glycocalyx studies often use far-red fluorophores to improve separation from autofluorescence and to enable multiplexing with other dyes in distinct channels. In practical assay development, the conjugate is used to generate fluorescence intensity distributions that reflect relative carbohydrate availability on the cell surface, supporting screening of glycan-modulating conditions and comparative binding studies.

3. Glycoprotein and Biomaterial Staining

Lectin from Phytolacca americana-ATTO 647N conjugate is applied to fluorescently label glycan-presenting proteins and to visualize carbohydrate-decorated biomaterials. Materials scientists and chemical biology teams use the lectin conjugate to confirm surface glycosylation patterns on coatings, hydrogels, and functionalized surfaces, and to monitor lectin-accessible glycan presentation after processing or storage. The far-red emission from ATTO 647N facilitates imaging on substrates that exhibit background fluorescence in the visible range, improving interpretability in fluorescence microscopy and plate-based endpoint assays.

4. Fluorescent Binding Assays

Lectin from Phytolacca americana-ATTO 647N conjugate is frequently incorporated into fluorescence-based binding assays that evaluate carbohydrate interactions and lectin competition. Researchers use the conjugate as a fluorescent reporter to monitor changes in lectin binding upon addition of soluble carbohydrates, glycan analogs, or glycoprotein competitors, enabling straightforward readouts in microplate formats. This approach is particularly useful in chemical biology studies where lectin-binding trends need to be tracked quantitatively under controlled buffer and incubation conditions.