IRDye® 800RS Carboxylate | CAS 918936-51-3

IRDye® 800RS Carboxylate is a near-infrared, red-emitting IRDye® 800RS fluorophore supplied as a carboxylate-bearing labeling dye for covalent attachment to biomolecules and materials. Its NIR emission supports low-background fluorescence workflows in microscopy, imaging systems, and quantitative fluorescence assays, where carboxylate-reactive conjugation strategies are used to generate stable fluorescent conjugates for tracking and detection.

1. Protein And Antibody Labeling

IRDye® 800RS Carboxylate is widely used for fluorescent labeling of proteins, including antibodies and antibody fragments, to create NIR imaging reagents for binding studies and immunoassays. Researchers commonly conjugate the dye to primary and secondary antibodies to generate imaging-compatible probes for western blot-style workflows, fluorescence-based binding assays, and quantitative signal readouts on NIR-capable detection platforms. The carboxylate functionality supports downstream conjugate construction workflows that produce dye-biomolecule conjugates used for target localization and comparative expression or binding experiments.

2. Fluorescence Imaging Tracers

IRDye® 800RS Carboxylate enables preparation of NIR fluorescent tracers for monitoring distribution and uptake of labeled biomolecules in cell-based imaging and in vitro imaging experiments. Molecular imaging teams and microscopy users employ IRDye® 800RS-conjugated reagents to visualize labeled ligands, binding partners, or carrier-associated components using NIR detection to reduce interference from visible fluorescence. Because the dye is provided in a conjugation-ready form, it is commonly incorporated into labeling pipelines for building imaging reagents used in time-course studies, co-localization experiments, and fluorescence readouts where NIR channels are advantageous.

3. Biomaterial And Surface Functionalization

IRDye® 800RS Carboxylate is used to fluorescently tag biomaterials, polymers, and engineered surfaces to enable visualization of coating uniformity, material localization, and surface-associated processes. Materials scientists and biomaterials labs incorporate IRDye® 800RS into functionalized constructs so that labeled scaffolds, membranes, or bulk materials can be tracked by NIR fluorescence during characterization and workflow validation. The carboxylate handle supports conjugation-oriented development of fluorescently labeled materials that are compatible with NIR imaging instrumentation used for assessing spatial distribution and retention of fluorescent labels on or within engineered substrates.

4. Nucleic Acid And Oligonucleotide Conjugates

IRDye® 800RS Carboxylate is also applied in fluorescent oligonucleotide labeling workflows to generate NIR probes for nucleic acid visualization and fluorescence-based nucleic acid assays. Molecular biology teams use IRDye® 800RS-labeled oligos as detection reagents in hybridization readouts and analytical gel or imaging formats that benefit from near-infrared signal collection. The dye's conjugation-ready carboxylate functionality supports building fluorescent nucleic acid conjugates used to track probe location, monitor hybridization workflows, and support multiplex-compatible NIR detection strategies when appropriate instrumentation is available.