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Fluorescein diacetate | CAS 596-09-8
| Catalog Number | F04-0038 |
| Category | Fluorescein FAM |
| Molecular Formula | C24H16O7 |
| Molecular Weight | 416.38 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Fluorescein diacetate (FDA) is a cell-permeant viability stain that produces green fluorescence upon esterase cleavage. It’s ideal for live/dead cell assays and plant viability tests.
Chemical Information
Product Specification
Application
Computed Properties
Literatures
Patents
| Synonyms | 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein; 3',6'-Bis(acetyloxy)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Fluorescein, diacetate; (6'-Acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate; 3',6'-Diacetylfluorescein; F 1303; FDA; Fluorescein 3',6'-diacetate; MFCD 5062; NSC 4726; NSC 667259 |
| Purity | >98% by HPLC |
| IUPAC Name | (6'-acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate |
| Canonical SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC=CC=C5C(=O)O3 |
| InChI | InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3 |
| InChIKey | CHADEQDQBURGHL-UHFFFAOYSA-N |
| Solubility | Soluble in Chloroform, DMSO, Ethanol, Water |
| Density | 1.47±0.1 g/cm3 |
| Appearance | Light yellow crystalline powder |
| Boiling Point | 604.7±55.0 °C at 760 mmHg |
| Melting Point | 205-206 °C |
| MDL Number | MFCD00005062 |
| Condition To Avoid | Air Sensitive |
| Storage | Store at -20 °C |
| Store Under Inert Gas | Store under inert gas |
| GHSHazardStatements | Not Classified Reported as not meeting GHS hazard criteria by 2 of 2 companies. For more detailed information, please visit ECHA C&L website. |
Fluorescein diacetate (FDA), a versatile non-fluorescent compound, finds widespread use in a variety of biological and medical assays. Here are four key applications of fluorescein diacetate:
Cell Viability Assays: An indispensable tool for assessing cell viability in diverse cell cultures, FDA undergoes enzymatic hydrolysis within viable cells to produce fluorescein, a vividly fluorescent compound. The resulting fluorescence serves as a means to quantify the percentage of live cells in a sample, making FDA a valuable asset in the realms of cell biology and toxicology studies.
Microbial Activity Detection: Utilized to gauge the metabolic activity of microorganisms in environmental and clinical samples, FDA serves as a marker for active microbial cells. These cells can enzymatically convert FDA into fluorescein, signaling their viability and activity. This application holds significant merit in fields such as soil microbiology, wastewater treatment research, and the examination of infectious agents.
Flow Cytometry: Within the domain of flow cytometry, FDA acts as a fluorescent dye for staining live cells for subsequent analysis. As viable cells metabolize FDA into fluorescein, they become distinguishable and quantifiable based on their fluorescence properties. This capability enables researchers to conduct intricate cell population studies and evaluate the individual cell health within a heterogeneous cell population.
Plant Viability Studies: In the field of plant sciences, FDA plays a pivotal role in assessing the viability and vitality of plant tissues and cells. By staining plant cells with FDA and detecting the resultant fluorescein production, researchers gain insights into the health status of plant tissues. This knowledge proves essential for investigations into plant growth, stress responses, and tissue culture techniques, offering a multifaceted view of plant physiology.
| XLogP3 | 3.6 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 7 |
| Rotatable Bond Count | 4 |
| Exact Mass | 416.08960285 g/mol |
| Monoisotopic Mass | 416.08960285 g/mol |
| Topological Polar Surface Area | 88.1Ų |
| Heavy Atom Count | 31 |
| Formal Charge | 0 |
| Complexity | 708 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
| PMID | Publication Date | Title | Journal |
|---|---|---|---|
| 27633002 | 2016-11-01 | Enalapril protects against myocardial ischemia/reperfusion injury in a swine model of cardiac arrest and resuscitation | International journal of molecular medicine |
| 22661061 | 2012-11-01 | Deciphering the metabolic capabilities of a lipase producing Pseudomonas aeruginosa SL-72 strain | Folia microbiologica |
| 22704207 | 2012-11-01 | Chlorination-induced cellular damage and recovery in marine microalga, Chlorella salina | Chemosphere |
| 22960224 | 2012-11-01 | Impacts of florfenicol on marine diatom Skeletonema costatum through photosynthesis inhibition and oxidative damages | Plant physiology and biochemistry : PPB |
| 22568927 | 2012-10-01 | Effects of vitrification cryoprotectant treatment and cooling method on the viability and development of buffalo oocytes after intracytoplasmic sperm injection | Cryobiology |
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-115058344-A | Decoy microrobot for removing SARS-CoV-2 and its variants in wastewater, its preparation method and application | 2022-08-05 |
| CN-115058344-B | Decoy microrobots for removing SARS-CoV-2 and its mutant strains in wastewater, its preparation method and application | 2022-08-05 |
| CN-115201302-A | Electrochemical sensor, preparation method and application thereof | 2022-07-04 |
| US-11548891-B1 | Quaternary ammonium salts of phenanthroindolizidine and phenanthroquinolizidine alkaloids as hypoxia-targeted anticancer agents | 2022-04-22 |
| CN-114848579-A | leech vibrio-containing composition | 2022-04-06 |
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