DyLight 3 CEP | CAS 182873-76-3

Name: DyLight 3 CEP
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	F02-0110
Category	Cyanine3
Molecular Formula	C58H70CIN4O4P
Molecular Weight	953.64

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Product Introduction

DyLight 3 CEP is a fluorescent dye used for oligonucleotide labeling.

Chemical Information
Product Specification
Application

Chemical Information

Related CAS	718594-66-2 (free base)
Synonyms	Cy3 MMTr CE Phosphoramidite; 1-[3-(4-monomethoxytrityloxy)propyl]-1'-[3-[(2-cyanoethyl)-(N,N-diisopropyl)phosphoramidityl]propyl]-3,3,3',3'-tetramethylindocarbocyanine chloride; Cyanine 3 Phosphoramidite
Purity	≥90%
IUPAC Name	3-[[di(propan-2-yl)amino]-[3-[2-[3-[1-[3-[(4-methoxyphenyl)-diphenylmethoxy]propyl]-3,3-dimethylindol-2-ylidene]prop-1-enyl]-3,3-dimethylindol-1-ium-1-yl]propoxy]phosphanyl]oxypropanenitrile;chloride
SMILES	CC(C)N(C(C)C)P(OCCC[N+]1=C(C(C2=CC=CC=C21)(C)C)C=CC=C3C(C4=CC=CC=C4N3CCCOC(C5=CC=CC=C5)(C6=CC=CC=C6)C7=CC=C(C=C7)OC)(C)C)OCCC#N.[Cl-]
InChI	InChI=1S/C58H70N4O4P.ClH/c1-44(2)62(45(3)4)67(65-42-21-38-59)66-43-23-40-61-53-31-19-17-29-51(53)57(7,8)55(61)33-20-32-54-56(5,6)50-28-16-18-30-52(50)60(54)39-22-41-64-58(46-24-12-10-13-25-46,47-26-14-11-15-27-47)48-34-36-49(63-9)37-35-48;/h10-20,24-37,44-45H,21-23,39-43H2,1-9H3;1H/q+1;/p-1
InChIKey	DQXAVRZQYYKELZ-UHFFFAOYSA-M

Product Specification

Storage

Store at -20°C

Application

DyLight 3 CEP is a DyLight 3-based fluorescent labeling reagent supplied in a reactive, bioconjugation-ready format for attaching the dye to biomolecules and engineered materials. Its visible-light emission supports common fluorescence microscopy and fluorescence-based readouts, while the reactive functionality enables incorporation into labeling workflows used in chemical biology, assay development, and molecular imaging reagent construction.

1. Protein Fluorescent Labeling

DyLight 3 CEP is used to generate fluorescently labeled proteins for binding and interaction studies, including antibody conjugates, affinity reagents, and protein tracking experiments in solution or on surfaces. Researchers typically incorporate DyLight 3 CEP into bioconjugation workflows to produce dye-tagged proteins compatible with standard fluorescence imaging instrumentation, enabling visualization of localization, relative abundance, and binding behavior in biochemical assays and imaging experiments.

2. Fluorescence Microscopy Tracing

DyLight 3 CEP supports cellular and biomaterial imaging workflows where a DyLight 3 fluorophore is used as a visible tracer. Chemical biology teams and microscopy users rely on DyLight 3 CEP-derived conjugates to follow labeled biomolecule distribution in fixed samples, to map reagent localization on functionalized substrates, and to support fluorescence imaging experiments that benefit from a bright, visible emission dye for multicolor experimental design.

3. Surface And Biomaterial Functionalization

DyLight 3 CEP is applied in materials research to introduce fluorescent tags onto polymers, nanoparticles, and biomaterial surfaces for imaging-guided characterization and tracking. In biomaterials and surface engineering workflows, DyLight 3 CEP-labeled components are used to visualize coating uniformity, monitor surface-associated binding events, and support fluorescence readouts in assays that interrogate material-biomolecule interactions under laboratory conditions.

4. Fluorescence-Based Bioassays

DyLight 3 CEP is commonly used to prepare fluorescent reagents for fluorescence measurements in plate-based or cuvette-based bioassays. By converting target-binding proteins or assay components into DyLight 3 conjugates, researchers can construct fluorescence readout formats for monitoring binding, immobilization, and reagent performance in screening and method-development studies where a visible fluorescent label is advantageous.