CypHer5 | CAS 312961-83-4

CypHer5 is a near-infrared fluorescent dye designed for fluorescence imaging workflows that benefit from reduced cellular autofluorescence and deeper tissue penetration. Its labeling chemistry is commonly used to generate stable fluorescent conjugates for biomolecule tracking, and its spectral output aligns with NIR imaging and fluorescence readout systems used in microscopy, imaging stations, and plate-based assays.

1. Fluorescence Microscopy Labeling

CypHer5 is frequently used by cell biology and chemical biology labs to visualize labeled biomolecules and to track conjugate localization in fluorescence microscopy experiments. Researchers prepare CypHer5-labeled proteins, peptides, or other targeting ligands to follow binding and trafficking behavior in cultured cells, while leveraging its NIR emission to improve contrast against background fluorescence in complex samples. The dye's conjugate format supports straightforward incorporation into staining or labeling panels for multi-channel imaging workflows where NIR signal separation is advantageous.

2. In Vivo Imaging Studies

CypHer5 is used in preclinical imaging-style research workflows to monitor fluorescently labeled biomolecules in whole-organism or ex vivo contexts where near-infrared detection is preferred. Imaging teams and translational research groups commonly employ CypHer5 conjugates to generate optical readouts from labeled probes, such as tracking distribution and persistence of fluorescent conjugates in biological matrices. The dye's NIR characteristics make it a practical choice for fluorescence imaging systems and optical imaging platforms that are configured for longer-wavelength excitation/emission detection.

3. Fluorescence-Based Bioassays

CypHer5 is also applied in fluorescence assay development where labeled reagents are used as reporters for binding, uptake, or interaction readouts in plate or cuvette formats. Assay developers generate CypHer5 conjugates to quantify fluorescence changes associated with assay-specific events, enabling robust workflow integration with standard fluorescence readers. This use case is common in research groups building fluorescence-based characterization assays for biomolecule conjugates, labeling reagents, and platform validation experiments that require NIR-compatible detection.