Cyanine3B azide | CAS 1914113-85-1

Cyanine3B azide is a cyanine-based fluorescent azide designed for copper-free click chemistry workflows, enabling efficient incorporation of a bright red-emitting dye into biomolecules, probes, and functional materials. Its azide handle supports bioorthogonal conjugation to alkyne-bearing partners, making it useful for fluorescent labeling, tracer construction, and imaging reagent development where dye attachment is required under mild conditions. The Cyanine3B chromophore provides strong fluorescence for microscopy and fluorescence-based readouts, while the azide functionality enables modular probe assembly.

1. Biomolecule Fluorescent Labeling

Cyanine3B azide is used by chemical biology and proteomics researchers to generate fluorescently labeled biomolecules through azide-alkyne click conjugation. In practice, it supports labeling of proteins, peptides, and other alkyne-functionalized targets for fluorescence imaging and binding studies, including experiments where dye loading is performed after biomolecule preparation. The cyanine dye core provides a convenient red fluorescence channel for multicolor experiments, and the azide handle helps standardize labeling workflows for probe libraries and reagent screening.

2. Fluorescent Probe Construction

Cyanine3B azide is widely adopted for building modular fluorescent probes and imaging reagents where a reactive dye is needed as a late-stage labeling component. Researchers incorporate the dye into alkyne-functional probe scaffolds to create conjugates for microscopy-based localization, tracking, and fluorescence reporting in complex biological matrices. This approach is especially useful for constructing probe variants that differ by targeting ligands or recognition elements while maintaining a consistent cyanine fluorophore readout, supporting iterative assay development and reagent optimization.

3. Biomaterials And Surface Functionalization

Cyanine3B azide supports fluorescent labeling of polymers, hydrogels, and biomaterial surfaces by click attachment to alkyne-functionalized materials. Materials scientists and biomaterials groups use the dye to visualize coating uniformity, track material-cell interactions, and generate fluorescent tags for scaffold characterization. Because the azide functionality enables conjugation to alkyne-bearing surfaces or linkers, Cyanine3B azide is commonly used to prepare imaging-ready materials for microscopy workflows without requiring harsh dye handling steps.

4. Nucleic Acid And Oligonucleotide Tagging

Cyanine3B azide is used in nucleic acid labeling workflows when alkyne-modified oligonucleotides are available for click conjugation, enabling fluorescent tagging for fluorescence imaging and nucleic-acid analysis development. Molecular biology teams incorporate the dye into oligonucleotide constructs to create fluorescent tracers for hybridization-related experiments and to support assay development where a red-emitting fluorophore is required as a reporter. This labeling strategy is also used to generate fluorescent standards and labeled components for method development in fluorescence-based nucleic acid workflows.