Ac-DEVD-AMC | 169332-61-0

Ac-DEVD-AMC is a synthetic peptide substrate widely used for studying caspase-3 activity, an enzyme crucial in apoptosis (programmed cell death). Here are some key applications of Ac-DEVD-AMC:

Apoptosis Research: Ac-DEVD-AMC is utilized in apoptosis research to measure caspase-3 activity, which is a key marker of cell apoptosis. When caspase-3 cleaves Ac-DEVD-AMC, it releases the fluorescent compound AMC, allowing researchers to quantify enzyme activity by fluorescence. This helps in understanding the mechanisms of apoptosis in various cell types and disease models.

Drug Screening: In pharmaceutical research, Ac-DEVD-AMC is employed in high-throughput screening assays to identify potential inhibitors or activators of caspase-3. By monitoring changes in fluorescence, researchers can evaluate the efficacy of novel compounds that may modify caspase activity. This is crucial for developing new therapeutic agents for diseases involving dysregulated apoptosis, such as cancer and neurodegenerative disorders.

Cancer Research: Ac-DEVD-AMC aids in studying the role of caspase-3 in cancer cell apoptosis, helping to elucidate how cancer cells evade programmed cell death. Researchers use this substrate to assess the apoptotic response of cancer cells to various treatments, such as chemotherapy and radiation. Understanding caspase-3 activity in cancer cells can lead to the development of more effective cancer therapies.

Neurodegenerative Disease Studies: Caspase-3 is implicated in the neuronal cell death seen in neurodegenerative diseases like Alzheimer’s and Parkinson’s. Ac-DEVD-AMC is used to study caspase-3 activity in neuronal cells and brain tissues affected by these diseases. This application provides insights into the molecular pathways driving neurodegeneration and helps in identifying potential therapeutic targets for these debilitating conditions.