Nuclear Yellow

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Nuclear Yellow

Nuclear Yellow | 74681-68-8

Catelog Number A19-0005
Category DNA Stains
Molecular Formula C25H28N7O2SCl3
Molecular Weight 596.96
Catalog Number Size Price Quantity
A19-0005 -- $--

Product Introduction

Nuclear yellow, as Hoechst S769121, belongs Hoechst stains, which were originally developed by Hoechst AG, which numbered all their compounds and are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. It is the long-wavelength tracer and is often combined with the popular retrograde tracer true blue for two-color neuronal mapping. It exhibits excitation/emission maxima ~335/495 nm when bound to DNA. In neuronal cells, it primarily stains the nucleus with yellow fluorescence. It is stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.

Chemical Information

Synonyms 4-[6-[6-(4-Methyl-1-Piperazinyl)-1H-Benzimidazol-2-Yl]-1H-Benzimidazol-2-Yl]Benzenesulfonamide Trihydrochloride;4-(5-(4-Methyl-1-Piperazinyl)(2,5'-Bi-1H-Benzimidazol)-2'-Yl)Benzenesulfonamide Trihydrochloride;Hoe-S769121;2-(4-Sulfamylphenyl)-6-[6-(4-methylpiperazino)-2-benzimidazolyl]benzimidazole trihydrochloride
Purity 98%
Shelf Life 2 month in rt, long time
IUPAC Name 4-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]benzenesulfonamide;trihydrochloride
Canonical SMILES CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC=C(C=C6)S(=O)(=O)N.Cl.Cl.Cl
InChI InChI=1S/C25H25N7O2S.3ClH/c1-31-10-12-32(13-11-31)18-5-9-21-23(15-18)30-25(28-21)17-4-8-20-22(14-17)29-24(27-20)16-2-6-19(7-3-16)35(26,33)34;;;/h2-9,14-15H,10-13H2,1H3,(H,27,29)(H,28,30)(H2,26,33,34);3*1H
InChI Key NZVGXJAQIQJIOY-UHFFFAOYSA-N
Solubility H2O: ≥ 6 mg/mL, DMSO: < 12.2 mg/mL
Appearance Light yellow powder
Boiling Point 813.6°C at 760 mmHg

Product Specification

Excitation 355 nm
Emission 495 nm
Storage -20°C Freezer, Protect from light
Application Nuclear yellow exhibits excitation/emission maxima ~335/495 nm when bound to DNA. In neuronal cells, it primarily stains the nucleus with yellow fluorescence.

Computed Properties

Hydrogen Bond Donor Count 6
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 4
Exact Mass 595.109077 g/mol
Monoisotopic Mass 595.109077 g/mol
Topological Polar Surface Area 132Ų
Heavy Atom Count 38
Formal Charge 0
Complexity 837
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 4
Compound Is Canonicalized Yes

Patents

Publication NumberTitlePriority Date
WO-2020161125-A1 Methods and systems for evaluation of immune cell infiltrate in stage iv colorectal cancer 2019-02-05
CN-113454458-A Methods and systems for assessing immune cell infiltration in stage IV colorectal cancer 2019-02-05
EP-3921648-A1 Methods and systems for evaluation of immune cell infiltrate in stage iv colorectal cancer 2019-02-05
US-2021373024-A1 Methods and systems for evaluation of immune cell infiltrate in stage iv colorectal cancer 2019-02-05
WO-2020104538-A1 Methods and systems for preparing and analyzing cellular samples for morphological characteristics and biomarker expression 2018-11-20
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