NADA-green

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NADA-green

NADA-green | 2253733-11-6

Catalog Number A21-0007
Category Fluorescent Probes for Imaging Bacteria
Molecular Formula C9H9N5O5.HCl
Molecular Weight 303.66
Catalog Number Size Price Quantity
A21-0007 -- $--

Product Introduction

NADA-green is a fluorescent D-amino acid that is incorporated into bacterial cell walls during synthesis and label peptidoglycans.

Chemical Information

Synonyms 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
Purity ≥98% by HPLC
IUPAC Name (2R)-2-amino-3-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]propanoic acid;hydrochloride
Canonical SMILES C1=C(C2=NON=C2C(=C1)[N+](=O)[O-])NCC(C(=O)O)N.Cl
InChI InChI=1S/C9H9N5O5.ClH/c10-4(9(15)16)3-11-5-1-2-6(14(17)18)8-7(5)12-19-13-8;/h1-2,4,11H,3,10H2,(H,15,16);1H/t4-;/m1./s1
InChI Key NKFVSANWNYYLLB-PGMHMLKASA-N
Solubility DMSO
  • Product Specification
  • Application
Excitation 450 nm
Emission 555 nm
Storage Store at -20°C

NADA-green, a fluorescent dye prevalent in biological and biochemical research, boasts versatile applications. Here are four key uses of NADA-green:

Immunofluorescence: Through the utilization of NADA-green, researchers can tag antibodies, enabling the visualization of specific proteins’ presence and distribution within cells and tissues using a fluorescence microscope. This technique is fundamental for scrutinizing protein localization and interactions, offering remarkable specificity and sensitivity in detecting target molecules amidst intricate biological samples.

Live Cell Imaging: Renowned for its low toxicity and robust fluorescence properties, NADA-green shines in live cell imaging endeavors. Scientists leverage this dye to track dynamic biological processes like cellular signaling, migration, and division in real-time, facilitating the exploration of cellular functions within their natural contexts and providing invaluable insights into cellular behavior and physiology.

Flow Cytometry: Embraced in flow cytometry settings, NADA-green serves to label and identify cells or particles based on specific attributes. This enables the simultaneous analysis of diverse parameters encompassing cell surface markers and intracellular proteins across a vast cell population. Such applications are indispensable for tasks ranging from immunophenotyping to cell sorting, proving pivotal in diagnostic and research landscapes.

Fluorescence-Based Assays: Within various fluorescence-based assays, NADA-green emerges as a stalwart tool for measuring biochemical activities such as enzyme activities, ligand-receptor interactions, and nucleic acid hybridization. Its vibrant fluorescence signature furnishes a dependable readout for quantifying these activities in high-throughput screening and analytical assays, thereby catalyzing drug discovery efforts and unraveling intricate molecular mechanisms.

Computed Properties

Hydrogen Bond Donor Count 4
Hydrogen Bond Acceptor Count 9
Rotatable Bond Count 4
Exact Mass 303.0370461 g/mol
Monoisotopic Mass 303.0370461 g/mol
Topological Polar Surface Area 160Ų
Heavy Atom Count 20
Formal Charge 0
Complexity 361
Isotope Atom Count 0
Defined Atom Stereocenter Count 1
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 2
Compound Is Canonicalized Yes
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