MeoSuc-Ala-Ala-Pro-Val-AMC | 72252-90-5

MeoSuc-Ala-Ala-Pro-Val-AMC, a synthetic substrate commonly utilized in protease activity assays, offers versatile applications:

Protease Activity Assays: Widely deployed to assess the activity of serine proteases, notably elastase, in diverse samples, MeoSuc-Ala-Ala-Pro-Val-AMC serves as a crucial tool. Through the cleavage of this substrate, proteases liberate a fluorescent reporter, enabling precise quantification of enzyme activity. This method is essential for scrutinizing protease functionality in biological and clinical specimens, shedding light on intricate enzymatic processes.

Drug Screening: In the realm of drug discovery, MeoSuc-Ala-Ala-Pro-Val-AMC plays a pivotal role in screening for potential protease inhibitors. Compounds hindering the cleavage of this substrate are earmarked as prospective drugs for conditions marked by heightened protease activity, such as inflammation and cancer. The remarkable sensitivity of this assay substrate renders it ideal for high-throughput screening, facilitating the identification of novel therapeutic agents.

Enzyme Kinetics: Scientists leverage MeoSuc-Ala-Ala-Pro-Val-AMC to explore the kinetic properties of proteases. By monitoring the pace of substrate cleavage, researchers unveil critical enzyme parameters like Km and Vmax. This data is indispensable for unraveling enzyme mechanisms and regulatory attributes, contributing to a deeper comprehension of enzymatic actions and behaviors.

Protease Characterization: A valuable asset in delineating the specificity and potency of diverse proteases, MeoSuc-Ala-Ala-Pro-Val-AMC aids in characterizing these enzymes. Each protease exhibits distinct cleavage patterns on this substrate, offering valuable insights into their substrate preferences and catalytic efficiency. This analytical approach aids in elucidating the roles of proteases across various physiological and pathological processes, enriching our understanding of the intricate interplay of these enzymes in biological systems.