Mag-indo-1 AM | CAS 130926-94-2

Intracellular Calcium Measurement: Mag-indo-1 AM is widely used for measuring intracellular calcium levels due to its high selectivity and sensitivity to Ca²⁺ ions. Once inside the cell, this membrane-permeable indicator is cleaved by esterases, trapping the active dye within the cytosol. Researchers utilize Mag-indo-1 AM in fluorescence-based assays to monitor real-time changes in intracellular calcium concentration, which is crucial for studying various cellular processes such as signal transduction, muscle contraction, and neurotransmitter release. This application is particularly valuable in neurobiology and cardiology, where understanding calcium dynamics is essential for elucidating mechanisms underlying neuronal activity and heart function.

Calcium Imaging in Live Cells: Mag-indo-1 AM is an effective tool for calcium imaging in live cells, providing high-resolution visualization of calcium fluxes in response to physiological and pharmacological stimuli. Its ratiometric properties allow researchers to accurately quantify calcium concentrations and assess cellular responses to external factors. This application is critical in studying calcium signaling pathways and their role in cellular communication, differentiation, and apoptosis. By enabling precise and dynamic imaging of calcium movements, Mag-indo-1 AM facilitates research into how cells regulate their functions in health and disease, making it indispensable in biomedical research.

Investigating Calcium Homeostasis: Maintaining calcium homeostasis is vital for cellular function, and disruptions in calcium balance are linked to various diseases, including neurodegenerative disorders and metabolic syndromes. Mag-indo-1 AM is used to investigate the mechanisms that control calcium homeostasis within cells. Researchers employ this indicator to study the function of calcium channels, pumps, and exchangers, as well as their regulation by different signaling molecules. This application helps in understanding how cells maintain stable calcium levels and how alterations in these processes contribute to pathophysiological conditions. Mag-indo-1 AM’s ability to provide detailed insights into calcium regulation makes it a powerful tool in the study of cellular homeostasis.

Drug Screening and Toxicology Studies: Mag-indo-1 AM is also used in drug screening and toxicology studies to evaluate the effects of compounds on intracellular calcium levels. Since calcium signaling plays a key role in various cellular processes, any disruption can indicate potential cytotoxicity or therapeutic efficacy of a compound. Researchers use this indicator in high-throughput screening assays to identify drugs that modulate calcium channels or influence calcium-dependent pathways. This application is particularly useful in the development of new pharmaceuticals targeting diseases such as cardiovascular disorders and neurological conditions. By providing reliable data on how compounds affect calcium dynamics, Mag-indo-1 AM aids in the identification and optimization of therapeutic candidates.