H-Gly-Gly-AMC | 208645-74-3

H-Gly-Gly-AMC, also referred to as N-[(benzyloxy)carbonyl]-glycylglycine 7-amino-4-methylcoumarin, serves as a fluorogenic substrate widely applied in diverse biochemical assays. Here are four key applications of H-Gly-Gly-AMC:

Protease Activity Assays: When evaluating protease activity, H-Gly-Gly-AMC emerges as a prime candidate. This substrate stands out for its efficacy in assessing enzymes like aminopeptidases and endopeptidases. Upon cleavage by these enzymes, the release of the AMC moiety elicits fluorescence, offering a quantitative gauge for scrutinizing enzyme kinetics and meticulously screening inhibitors.

Drug Discovery: In the realm of drug discovery, H-Gly-Gly-AMC assumes a pivotal role in high-throughput screening assays geared towards uncovering potential protease inhibitors. Researchers can swiftly evaluate numerous compounds to measure their efficacy in inhibiting enzyme activity. This expedites the identification of promising drug candidates, propelling them through the research pipeline.

Diagnostic Applications: By utilizing H-Gly-Gly-AMC in diagnostic tests, researchers can pinpoint specific enzyme activities in biological samples, aiding in diagnosing conditions linked to anomalous protease activity, such as certain cancers or metabolic disorders. These assays furnish vital data for clinical decision-making, optimizing patient care.

Biochemical Research: Serving as a cornerstone tool in foundational biochemical research, H-Gly-Gly-AMC empowers scientists to delve deeply into protease mechanisms and substrate specificity. By analyzing how various proteases interact with this substrate, researchers can unearth profound insights into enzyme function and regulation, significantly enriching our comprehension of protein processing and turnover across diverse biological contexts.