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Specific Fluorescent Stains
PCR & Molecular Biology
RNA/DNA Labeling
Fluorescein-12-dCTP

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Fluorescein-12-dCTP

Catalog Number	A07-0040
Category	RNA/DNA Labeling
Molecular Formula	C39H40N5O20P3
Molecular Weight	991.68

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Product Introduction

Fluorescein-12-dCTP is a fluorescent nucleotide analog widely used in biomedical field. This product is commonly employed in DNA labeling and detection techniques, such as DNA microarrays and fluorescence in situ hybridization (FISH). It enables the visualization and tracking of specific DNA sequences is assisting in the research of genetic disorders and diseases.

Chemical Information
Product Specification
Application

Synonyms	Fluorescein-X-5-Propargylamino-dCTP; 5/6-Fluorescein-X-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
Purity	≥ 95 % by HPLC
pH	7.5 ±0.5
Appearance	yellow-orange solution in Tris-HCl

Spectroscopic Properties	λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Storage	store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.

Fluorescein-12-dCTP, a fluorescently labeled nucleotide widely harnessed in molecular biology and biotechnology, boasts a multitude of applications. Here are four key applications of Fluorescein-12-dCTP:

DNA Sequencing: By integrating Fluorescein-12-dCTP into DNA during sequencing reactions, researchers can witness real-time visualization of nucleotide incorporation. The fluorescence emanating from this labeled nucleotide aids in the precise identification of nucleotide bases within the DNA strand undergoing analysis, rendering it an indispensable tool in various genome sequencing initiatives and personalized medicine endeavors.

Fluorescent In Situ Hybridization (FISH): Integrated into FISH techniques, Fluorescein-12-dCTP acts as the labeling agent for DNA probes that hybridize to specific sequences within chromosomes. The resulting fluorescent signals from these probes aid in the identification and localization of specific genetic material within cellular preparations. This application proves particularly valuable in diagnosing genetic disorders and detecting chromosomal anomalies.

PCR Labeling: Within the landscape of Polymerase Chain Reaction (PCR), Fluorescein-12-dCTP can be incorporated into amplicons to generate fluorescently labeled DNA fragments. These labeled fragments can be leveraged in various downstream procedures, such as gel electrophoresis, microarrays, or real-time PCR assays. This methodology enhances the sensitivity and specificity of DNA detection processes, offering improved precision and accuracy.

Molecular Diagnostics: In the realm of molecular diagnostics, Fluorescein-12-dCTP assumes a pivotal role in the development of diagnostic assays for pathogen detection and genetic mutation identification. The fluorescence emitted by this nucleotide facilitates the straightforward and precise quantification of target DNA sequences, enhancing the reliability of diagnostic tests. This application holds critical importance in clinical settings for the timely and accurate diagnosis of various diseases.